OBJECTIVE: To observe the effects of cigarette smoke extract (CSE) on the proliferation and the expression of TGF-beta(1) and EGF in human embryonic lung fibroblasts (HELF). METHODS: The cultures of HELF were incubated with CSE (1:50, 1:25 and 1:10) for examining the effects of CSE on the proliferation and the proliferating cell nuclear antigen (PCNA) levels in HELF. The total RNA was extracted from the cells incubated with CSE at different doses. The expression levels of TGF-beta(1) mRNA were evaluated by reverse transcription-polymerase chain reaction (RT-PCR). The mRNA levels of TGF-beta(1) were corrected by GAPDH transcripts. TGF-beta(1) and EGF protein levels were determined by immunocytochemistry. RESULTS: The proliferation of HELF was inhibited by CSE in a dose-dependent manner. CSE also decreased the PCNA levels of HELF at all doses (P < 0.05). CSE stimulated TGF-beta(1) mRNA and protein expression of HELF at lower concentrations (1:50 and 1:25, P < 0.05). TGF-beta(1) mRNA and protein production were not increased by CSE at a higher concentration (1:10, P > 0.05). Decreased expression of EGF protein was observed in HELF treated by CSE (1:25 and 1:10, P < 0.05). CONCLUSIONS: CSE can inhibit the proliferation of HELF and the effect may involve the abnormal expression of TGF-beta(1) and EGF in HELF.
|Original language||English (US)|
|Number of pages||4|
|Journal||Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases|
|State||Published - Jan 2004|