Abstract
16s rRNA gene sequence analysis is applied in the semi-continuous fermentation of Chlorella pyrenoidosa to monitor changes in the microbial community of HPB (hydrogen-producing bacteria). The microbial community of HPB on the 4th day is mainly composed of one genus (Clostridium) including one primary species (Clostridium butyricum). The microbial community of HPB on the 22nd day is mainly composed of three genera (Clostridium, Sporanaerobacter and Eubacterium) including three primary species (Clostridium acetireducens, Clostridium cochlearium and Sporanaerobacter acetigenes). Fermentation results show that production of hydrogen and soluble metabolites by the simple microbial community is unstable with fluctuating pH values at earlier stages (days 1-4). By contrast, the production of hydrogen (51.7-62.0mL/g-total volatile solids) and soluble metabolites (acetate/butyrate: 0.4-0.5mol/mol) by complex microbial communities is fairly stable with a steady pH value of approximately 5.9 at later stages of fermentation (days 11-22).
| Original language | English (US) |
|---|---|
| Pages (from-to) | 982-988 |
| Number of pages | 7 |
| Journal | Energy |
| Volume | 68 |
| DOIs | |
| State | Published - Apr 15 2014 |
| Externally published | Yes |
Bibliographical note
Funding Information:This study was supported by the National Natural Science Foundation of China ( 51176163 ), National High Technology R&D Program of China ( 2012AA050101 ), International Sci. & Tech. Cooperation Program of China ( 2012DFG61770 and 2010DFA72730 ), National Key Technology R&D Program of China ( 2011BAD14B02 ), Program for New Century Excellent Talents in University (NCET-11-0446), Specialized Research Fund for the Doctoral Program of Higher Education ( 20110101110021 ), Science and Technology Project of Guangxi Province (1346011-1).
Keywords
- Hydrogen
- Microalgae
- Microbial community
- Semi-continuous fermentation
- Soluble metabolites