Abstract
We have studied normal and transformed cultured human fibroblasts by ultrastructural immunocytochemistry with antibodies to fibronectin (the large external transformation-sensitive glycoprotein). Human fibroblasts at low density have very little extracellular material organized into a filamentous form. A diffuse membrane component of low-density fibroblasts reacts with fibronectin antibodies. As normal fibroblasts grow to confluence, an extensive extracellular filamentous matrix forms. The filaments are 15 to 20 nm in diameter, lack periodicity, and frequently occur in a meshwork. Antibody to fibronectin reacts with these filaments as well as with a diffusely distributed membrane component. Cultures of SV40-transformed human fibroblasts lack the extracellular filamentous matrix reacting with fibronectin antibodies; however, fibronectin is detected on plasma membranes. These studies indicate that a major difference between normal and transformed human fibroblasts is the failure of transformed fibroblasts to form a fibronectin-containing extracellular filamentous matrix.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 4618-4623 |
| Number of pages | 6 |
| Journal | Cancer Research |
| Volume | 38 |
| Issue number | 12 |
| State | Published - Dec 1978 |
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Effects of Cell Density and Transformation on the Formation of a Fibronectin Extracellular Filamentous Matrix on Human Fibroblasts. / Furcht, Leo T; Mosher, D. F.; Wendelschafer-Crabb, G.
In: Cancer Research, Vol. 38, No. 12, 12.1978, p. 4618-4623.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effects of Cell Density and Transformation on the Formation of a Fibronectin Extracellular Filamentous Matrix on Human Fibroblasts
AU - Furcht, Leo T
AU - Mosher, D. F.
AU - Wendelschafer-Crabb, G.
PY - 1978/12
Y1 - 1978/12
N2 - We have studied normal and transformed cultured human fibroblasts by ultrastructural immunocytochemistry with antibodies to fibronectin (the large external transformation-sensitive glycoprotein). Human fibroblasts at low density have very little extracellular material organized into a filamentous form. A diffuse membrane component of low-density fibroblasts reacts with fibronectin antibodies. As normal fibroblasts grow to confluence, an extensive extracellular filamentous matrix forms. The filaments are 15 to 20 nm in diameter, lack periodicity, and frequently occur in a meshwork. Antibody to fibronectin reacts with these filaments as well as with a diffusely distributed membrane component. Cultures of SV40-transformed human fibroblasts lack the extracellular filamentous matrix reacting with fibronectin antibodies; however, fibronectin is detected on plasma membranes. These studies indicate that a major difference between normal and transformed human fibroblasts is the failure of transformed fibroblasts to form a fibronectin-containing extracellular filamentous matrix.
AB - We have studied normal and transformed cultured human fibroblasts by ultrastructural immunocytochemistry with antibodies to fibronectin (the large external transformation-sensitive glycoprotein). Human fibroblasts at low density have very little extracellular material organized into a filamentous form. A diffuse membrane component of low-density fibroblasts reacts with fibronectin antibodies. As normal fibroblasts grow to confluence, an extensive extracellular filamentous matrix forms. The filaments are 15 to 20 nm in diameter, lack periodicity, and frequently occur in a meshwork. Antibody to fibronectin reacts with these filaments as well as with a diffusely distributed membrane component. Cultures of SV40-transformed human fibroblasts lack the extracellular filamentous matrix reacting with fibronectin antibodies; however, fibronectin is detected on plasma membranes. These studies indicate that a major difference between normal and transformed human fibroblasts is the failure of transformed fibroblasts to form a fibronectin-containing extracellular filamentous matrix.
UR - http://www.scopus.com/inward/record.url?scp=0018182738&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0018182738&partnerID=8YFLogxK
M3 - Article
C2 - 719641
AN - SCOPUS:0018182738
VL - 38
SP - 4618
EP - 4623
JO - Journal of Cancer Research
JF - Journal of Cancer Research
SN - 0099-7013
IS - 12
ER -