Abstract
Objective: To directly examine the effects of carnosine on neuronal excitation and inhibition in rat hippocampus in vivo. Methods: Artificial cerebrospinal fluid with carnosine was directly administrated over the exposed rat hippocampus. The changes of neuron activity in the CA1 region of hippocampus were evaluated by orthodromically- and antidromically-evoked potentials, as well as paired-pulse stimulation paradigm. Results: In both orthodromic and antidromic response potentials, carnosine transformed population spikes (PSs) with single spike into epileptiform multiple spikes. In addition, similar to the effect of γ-aminobutyric acidA (GABAA) antagonist picrotoxin, carnosine decreased paired-pulse stimulating depression significantly. However, no significant change was observed in the spontaneous field potentials during the application of carnosine. Conclusion: The results indicate a disinhibition-induced excitation effect of carnosine on the CA1 pyramidal neurons. It provides important information against the application of carnosine as a potential anticonvulsant in clinical treatment.
Original language | English (US) |
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Pages (from-to) | 505-511 |
Number of pages | 7 |
Journal | Journal of Zhejiang University: Science B |
Volume | 10 |
Issue number | 7 |
DOIs | |
State | Published - 2009 |
Externally published | Yes |
Bibliographical note
Funding Information:* Project (Nos. 30570585 and 30770548) supported by the National Natural Science Foundation of China
Keywords
- Carnosine
- Epileptic seizure
- Evoked potential
- Hippocampus