Effects of blue light and phenotype on anthocyanin accumulation in accessions and cultivars of rough bluegrass

Dominic P. Petrella, James D. Metzger, Joshua J. Blakeslee, Edward J. Nangle, David S. Gardner

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Anthocyanins are increasingly being used as natural alternatives in medicinal, food, and industrial products. However, production of anthocyanin extract is often inefficient due to agronomic limitations. On the other hand, the use of turfgrasses for anthocyanin production has been suggested to increase yield twofold. Rough bluegrass (Poa trivialis L.) cultivar ‘Havana’ has been shown to increase anthocyanin content by 117-fold under high light treatment, exhibiting concentrations similar to current anthocyanin sources, and could be an alternative source of anthocyanin. The objectives of this research were to evaluate variation in anthocyanin content in 20 accessions and five cultivars of rough bluegrass treated with blue light and to evaluate phenotypes associated with anthocyanin content in this species to help develop germplasm screening tools. Following blue light treatment, rough bluegrass US cultivars ‘Laser’, ‘ProAm’, ‘Sabre’, ‘Colt’, Havana, and accessions originating from Germany produced statistically greater quantities of anthocyanin compared with other accessions. Phenotypes associated with anthocyanins, including total phenolic content, total flavonoid content, specific leaf area, leaf cuticular wax, and the chlorophyll:carotenoid ratio, were variable for all accessions and cultivars. However, accessions producing greater quantities of anthocyanin exhibited less cuticular wax. Results of a principle component analysis showed that there was a significant negative correlation between leaf cuticular wax and anthocyanin content. These data demonstrate that phenotypes associated with increased anthocyanin content, especially leaf cuticular waxes, could serve as screening tools for evaluating other turfgrasses as anthocyanin sources and may therefore help to maximize industrial anthocyanin production.

Original languageEnglish (US)
Pages (from-to)S-209-S-217
JournalCrop Science
StatePublished - Jul 1 2017

Bibliographical note

Funding Information:
This work is part of a thesis by a senior author in partial fulfillment of the requirements for the Ph.D. degree at the Ohio State University (Columbus, OH). Salaries and research support provided in part by State and Federal funds appropriated to the Ohio Agricultural Research and Development Center, The Ohio State University (Wooster, OH). This is journal article number HCS-16-15. We thank the James B. and Harriet Beard Graduate Fund for salary and research support. Thanks also to J.R. Simplot Company (Boise, ID) for seed used in these experiments.

Publisher Copyright:
© Crop Science Society of America. All rights reserved.

Copyright 2017 Elsevier B.V., All rights reserved.


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