Effects of anesthetic agents on bile pigment excretion in the rat

Glenn R. Gourley, William Mogilevsky, Richard A. Arend, Frank L. Siegel, Gerard B. Odell

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Anesthesia‐induced alterations in bilirubin conjugation were studied. Rats were fitted with bile duct and jugular vein catheters while anesthetized with diethyl ether, ketamine or pentobarbital. As anesthesia abated, bile was collected for the next 5 hr and analyzed for flow rate, total bilirubin excretion and bilirubin glucuronide composition. The high‐performance liquid chromatography method used allowed direct analysis of bile without derivatization or extraction. Ether anesthesia was associated with a reversible suppression of diglucuronide formation and total bilirubin excretion, with reciprocal monoglucuronide changes. Bile flow and pigment excretion were variable with ketamine. Pentobarbital provided the most uniform excretion data, although the ratio of C‐8:C‐12 monoglucuronide varied with all drugs. These data are consistent with recently reported drug‐induced alterations in hepatic uridine diphosphoglucuronic acid concentration and support the hypothesis that alterations in this substrate concentration are capable of influencing rates of hepatic glucuronide formation.

Original languageEnglish (US)
Pages (from-to)610-614
Number of pages5
JournalHepatology
Volume5
Issue number4
DOIs
StatePublished - 1985

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