Effects of acute and chronic STZ-induced diabetes on clock gene expression and feeding in the gastrointestinal tract

Jonathon Bostwick, Diane Nguyen, Germaine G Cornelissen-Guillaume, Franz Halberg, Willemijntje A. Hoogerwerf

Research output: Contribution to journalArticlepeer-review

24 Scopus citations


Diabetes may shift clock gene expression within peripheral organs. However, little is known about the effect of diabetes on the gastrointestinal molecular clock. We therefore investigated the effect of diabetes on gastrointestinal clock gene expression. As peripheral clock gene expression is strongly driven by food intake, we also determined the effect of STZ-induced diabetes on patterns of food intake. The effects of acute (1 week) and chronic (12 weeks) STZ-induced diabetes on period (per) genes in the stomach body, proximal and distal colon, liver, kidney, and lung of C57BL/6J mice were assessed using real-time polymerase chain reaction. Food intake studies were completed using automated feeding equipment. Rhythmicity in expression of per2 and per3 persisted in all organs. However, per2 and per3 expression of STZ-injected mice was generally phase delayed within the gastrointestinal tract but not within the kidney or lung as compared with vehicle-injected mice. The phase delay was most pronounced for per2 in the proximal colon at 12 weeks. Food intake was rhythmic with larger circadian amplitude for diabetic mice than for control mice. Thus, STZ-induced diabetes differentially alters peripheral per expression. STZ-induced diabetes does not alter the circadian phase of food intake. Alterations in clock gene expression in a mouse model of diabetes are most pronounced in those organs that are intimately associated with food processing and metabolism.

Original languageEnglish (US)
Pages (from-to)203-213
Number of pages11
JournalMolecular and cellular biochemistry
Issue number1-2
StatePublished - May 2010

Bibliographical note

Funding Information:
Acknowledgment This study is supported by R21 DK074477 and the University of Michigan Gastrointestinal Peptide Research Program (WAH). Diane Nguyen is a recipient of an AGA Student Fellowship Award.


  • Circadian biology
  • Clock genes
  • Diabetes
  • Gastrointestinal tract
  • Intestine
  • Motility
  • Streptozotocin


Dive into the research topics of 'Effects of acute and chronic STZ-induced diabetes on clock gene expression and feeding in the gastrointestinal tract'. Together they form a unique fingerprint.

Cite this