Effects of acute and chronic morphine treatments on calcium localization and binding in brain

H. Yamamoto, R. A. Harris, H. H. Loh, E. L. Way

Research output: Contribution to journalArticle

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Abstract

Acute subcutaneous injection of 25 mg/kg of morphine in rats decreased synaptosomal CA++ levels by 29% without altering the Ca++ content of other subcellular fractions. In contrast, chronic morphine treatment of mice or rats by pellet implantation selectively increased synaptosomal Ca++ levels by almost 100%. This increase in Ca++ induced by morphine was blocked by simultaneous chronic administration of naloxone. The binding of low concentrations (10-7-10-5 M) of 45Ca++ to synaptic plasma membranes was increased by acute morphine treatment and decreased by chronic administration. The binding of higher concentrations (10-3 M) of 45Ca++ to synaptic vesicles was also increased by acute morphine treatment and decreased by chronic treatment. Changes in binding were not observed with other subcellular fractions. It is suggested that these highly selective changes in Ca++ levels and binding may represent mechanisms by which acute morphine treatment interferes with synaptic transmission and by which chronic administration overcomes these effects, resulting in tolerance and dependence.

Original languageEnglish (US)
Pages (from-to)255-264
Number of pages10
JournalJournal of Pharmacology and Experimental Therapeutics
Volume205
Issue number2
StatePublished - Dec 1 1978

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Morphine
Calcium
Brain
Subcellular Fractions
Therapeutics
Synaptic Membranes
Synaptic Vesicles
Subcutaneous Injections
Naloxone
Synaptic Transmission
Cell Membrane

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Effects of acute and chronic morphine treatments on calcium localization and binding in brain. / Yamamoto, H.; Harris, R. A.; Loh, H. H.; Way, E. L.

In: Journal of Pharmacology and Experimental Therapeutics, Vol. 205, No. 2, 01.12.1978, p. 255-264.

Research output: Contribution to journalArticle

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