Effects of a SARS-associated coronavirus vaccine in monkeys

Wentao Gao, Azaibi Tamin, Adam Soloff, Leonardo D'Aiuto, Edward Nwanegbo, Paul D. Robbins, William J. Bellini, Simon Barratt-Boyes, Andrea Gambotto

Research output: Contribution to journalArticlepeer-review

199 Scopus citations


The causative agent of severe acute respiratory syndrome (SARS) has been identified as a new type of coronavirus. Here, we have investigated the ability of adenoviral delivery of codon-optimised SARS-CoV strain Urbani structural antigens spike protein S1 fragment, membrane protein, and nucleocapsid protein to induce virus-specific broad immunity in rhesus macaques. We immunised rhesus macaques intramuscularly with a combination of the three Ad5-SARS-CoV vectors or a control vector and gave a booster vaccination on day 28. The vaccinated animals all had antibody responses against spike protein S1 fragment and T-cell responses against the nucleocapsid protein. All vaccinated animals showed strong neutralising antibody responses to SARS-CoV infection in vitro. These results show that an adenoviral-based vaccine can induce strong SARS-CoV-specific immune responses in the monkey, and hold promise for development of a protective vaccine against the SARS causal agent.

Original languageEnglish (US)
Pages (from-to)1895-1896
Number of pages2
Issue number9399
StatePublished - Dec 6 2003
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported by National Heart Lung and Blood Institute, Program of Excellence in Gene Therapy supplement Grants U01 HL66949–01S1 to A Gambotto. The authors thank K Okada and H Sun for assistance with adenoviral preparation. The sponsors of the study had no role in study design, data collection, data analysis, data interpretation, or in the writing of the report.

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