Effect of stereoconfiguration on ripple phases (Pβ′) of dipalmitoylphosphatidylcholine

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Abstract

Mixtures of sn-1 (d) and sn-3 (l) enantiomers of fully hydrated dipalmitoylphosphatidylcholine (DPPC) were studied with differential scanning calorimetry and freeze-fracture microscopy. The pretransition temperature of racemic mixtures of DPPC was 1.8 C° below that of either pure sn-1 or sn-3 enantiomers, which had similar pretransition temperatures. The main transition temperature of racemic mixtures was also depressed, but to a lesser extent, 0.8 C°. Freeze-fracture images of liposomes of sn-1, sn-3, and racemic mixtures of DPPC frozen from the Pβ′ phase showed well-defined ripples of wavelength 13 nm. Lipid stereoconfiguration had no effect on ripple wavelength, configuration or amplitude, or on the number and nature of surface defects.

Original languageEnglish (US)
Pages (from-to)235-243
Number of pages9
JournalBBA - Biomembranes
Volume946
Issue number2
DOIs
StatePublished - Dec 22 1988

Keywords

  • Dipalmitoylphosphatidylcholine
  • Liposome
  • Membrane structure
  • Ripple phase
  • Stereo chemistry

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