Effect of starvation and insulin treatment on glycogen synthase D and synthase D phosphatase activity in rat heart

Mary C Gannon, Agnes W H Tan, frank q Nuttall

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

We have previously shown that synthase phosphatase activity was decreased in starved animals and was rapidly restored by insulin administration (1). In order to determine whether the decreased phosphatase activity was due to a decrease in phosphatase enzyme per se or to a change in the substrate, synthase D, phosphatase activity has been determined using purified synthase D substrate. Using purified heart or liver synthase D, phosphatase activity was lower in extracts from starved animals than in fed animals. Insulin administration rapidly increased phosphatase activity in extracts from the starved animals. The total amount of endogenous synthase D which was convertible to synthase I was lower in extracts from starve animals, but this was rapidly increased within 15 minutes following insulin administration. These data suggest that starvation and insulin have a direct effect on the phosphatase enzyme activity per se and probably on the substrate suitability of synthase D as well.

Original languageEnglish (US)
Pages (from-to)31-34
Number of pages4
JournalMolecular and cellular biochemistry
Volume34
Issue number1
DOIs
StatePublished - Jan 1 1981

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