TY - JOUR
T1 - Effect of serum proteins and osteoblasts on the surface transformation of a calcium phosphate coating
T2 - A physicochemical and ultrastructural study
AU - Radin, S.
AU - Ducheyne, P.
AU - Berthold, P.
AU - Decker, S.
PY - 1998/2
Y1 - 1998/2
N2 - Changes occurring at the surface of a calcium phosphate coating when in contact with osteoblasts versus those in acellular solutions were analyzed. The coating studied is one with a well-documented extensive effect on short- term bone growth stimulation. Precipitates associated with original crystals and organized in a weblike structure were observed after a 3-week culture with osteoblasts. The precipitates were identified as carbonated hydroxyapatite (c-HA). In contrast, no significant surface changes were detected after immersion in an acellular serum-containing solution. However, in an acellular serum-free solution simulating the ionic composition of plasma, precipitates, identified as c-HA, were abundantly formed. Dissolution of the original coating preceded precipitation. The data support the hypothesis that dissolution of synthetic calcium phosphate ceramics is an initial step in their transformation to a biologically equivalent apatite, and suggest that both solution-mediated (dissolution-precipitation) and cell- mediated mechanisms are involved in the surface transformation.
AB - Changes occurring at the surface of a calcium phosphate coating when in contact with osteoblasts versus those in acellular solutions were analyzed. The coating studied is one with a well-documented extensive effect on short- term bone growth stimulation. Precipitates associated with original crystals and organized in a weblike structure were observed after a 3-week culture with osteoblasts. The precipitates were identified as carbonated hydroxyapatite (c-HA). In contrast, no significant surface changes were detected after immersion in an acellular serum-containing solution. However, in an acellular serum-free solution simulating the ionic composition of plasma, precipitates, identified as c-HA, were abundantly formed. Dissolution of the original coating preceded precipitation. The data support the hypothesis that dissolution of synthetic calcium phosphate ceramics is an initial step in their transformation to a biologically equivalent apatite, and suggest that both solution-mediated (dissolution-precipitation) and cell- mediated mechanisms are involved in the surface transformation.
KW - Calcium phosphates
KW - Ceramics
KW - In vitro
KW - Surface reactions
KW - Surface transformations
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U2 - 10.1002/(SICI)1097-4636(199802)39:2<234::AID-JBM10>3.0.CO;2-D
DO - 10.1002/(SICI)1097-4636(199802)39:2<234::AID-JBM10>3.0.CO;2-D
M3 - Article
C2 - 9457553
AN - SCOPUS:0032007951
SN - 1552-4973
VL - 39
SP - 234
EP - 243
JO - Journal of Biomedical Materials Research
JF - Journal of Biomedical Materials Research
IS - 2
ER -