Effect of serum proteins and osteoblasts on the surface transformation of a calcium phosphate coating: A physicochemical and ultrastructural study

S. Radin, P. Ducheyne, P. Berthold, S. Decker

Research output: Contribution to journalArticle

46 Scopus citations

Abstract

Changes occurring at the surface of a calcium phosphate coating when in contact with osteoblasts versus those in acellular solutions were analyzed. The coating studied is one with a well-documented extensive effect on short- term bone growth stimulation. Precipitates associated with original crystals and organized in a weblike structure were observed after a 3-week culture with osteoblasts. The precipitates were identified as carbonated hydroxyapatite (c-HA). In contrast, no significant surface changes were detected after immersion in an acellular serum-containing solution. However, in an acellular serum-free solution simulating the ionic composition of plasma, precipitates, identified as c-HA, were abundantly formed. Dissolution of the original coating preceded precipitation. The data support the hypothesis that dissolution of synthetic calcium phosphate ceramics is an initial step in their transformation to a biologically equivalent apatite, and suggest that both solution-mediated (dissolution-precipitation) and cell- mediated mechanisms are involved in the surface transformation.

Original languageEnglish (US)
Pages (from-to)234-243
Number of pages10
JournalJournal of Biomedical Materials Research
Volume39
Issue number2
DOIs
StatePublished - Feb 1998

Keywords

  • Calcium phosphates
  • Ceramics
  • In vitro
  • Surface reactions
  • Surface transformations

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