Effect of porcine parvovirus vaccination on the development of PMWS in segregated early weaned pigs coinfected with type 2 porcine circovirus and porcine parvovirus

T. Opriessnig; M. Fenaux; S. Yu; R. B. Evans; D. Cavanaugh; J. M. Gallup; F. J. Pallares; E. L. Thacker; K. M. Lager; X. J. Meng; P. G. Halbur

doi:10.1016/j.vetmic.2003.11.006

Effect of porcine parvovirus vaccination on the development of PMWS in segregated early weaned pigs coinfected with type 2 porcine circovirus and porcine parvovirus

T. Opriessnig, M. Fenaux, S. Yu, R. B. Evans, D. Cavanaugh, J. M. Gallup, F. J. Pallares, E. L. Thacker, K. M. Lager, X. J. Meng, P. G. Halbur

Research output: Contribution to journal › Article › peer-review

76 Scopus citations

Abstract

The objectives of this study were to determine if coinfection of segregated early weaned (SEW) pigs with porcine circovirus type 2 (PCV2) and porcine parvovirus (PPV) induces an increase in the incidence of post-weaning multisystemic wasting syndrome (PMWS) compared to singular PCV2 infection, and to determine if vaccination against PPV protects pigs against PMWS associated with PCV2/PPV coinfection in SEW pigs. Seventy, 3-week-old, SEW pigs were randomly assigned to one of the five groups. Pigs in group 1 (n=14) served as the negative controls, group 2 pigs (n=14) were inoculated with PCV2, group 3 pigs (n=12) were inoculated with PPV, groups 4 (n=16) and 5 (n=14) pigs were inoculated with both PCV2 and PPV. Pigs in groups 1-3 and 5 were vaccinated with two doses of a killed parvovirus-leptospira-erysipelothrix (PLE) vaccine prior to inoculation. The PCV2/PPV-coinfected pigs (groups 4 and 5) had significantly (P<0.05) higher and more persistent fevers than the singular PCV2-infected pigs. One pig in each of the coinfected groups developed clinical disease (fever, respiratory disease, jaundice, weight loss) consistent with PMWS. Lymphoid depletion was significantly (P<0.05) more severe in the dually-infected pigs at 42 days post-inoculation (DPI). Vaccinated, coinfected pigs (group 5) remained viremic significantly (P<0.05) longer and had higher copy numbers of genomic PCV2 DNA in sera at 28, 35, and 42 DPI compared to the unvaccinated coinfected pigs (group 4). PPV-viremia was detected only in the unvaccinated group 4 pigs. PLE-vaccination prevented PPV-viremia but did not prevent clinical PMWS or reduce the severity of lymphoid depletion in PCV2/PPV-coinfected pigs. Evidence of increased incidence of clinical PMWS due to vaccination was not observed in this model.

Original language	English (US)
Pages (from-to)	209-220
Number of pages	12
Journal	Veterinary Microbiology
Volume	98
Issue number	3-4
DOIs	https://doi.org/10.1016/j.vetmic.2003.11.006
State	Published - Mar 5 2004
Externally published	Yes

Bibliographical note

Funding Information:
The authors thank Ann Vorwald for preparing the PPV-inoculum and for helpful advice with PPV serology. We also thank Pete Thomas and Marlin Hoogland for assistance with animal work. The study was funded by a grant from Pfizer Animal Health, Inc.

Keywords

Porcine circovirus type 2
Porcine parvovirus
Post-weaning multisystemic wasting syndrome
Vaccination

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.vetmic.2003.11.006

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Opriessnig, T., Fenaux, M., Yu, S., Evans, R. B., Cavanaugh, D., Gallup, J. M., Pallares, F. J., Thacker, E. L., Lager, K. M., Meng, X. J., & Halbur, P. G. (2004). Effect of porcine parvovirus vaccination on the development of PMWS in segregated early weaned pigs coinfected with type 2 porcine circovirus and porcine parvovirus. Veterinary Microbiology, 98(3-4), 209-220. https://doi.org/10.1016/j.vetmic.2003.11.006

Opriessnig, T, Fenaux, M, Yu, S, Evans, RB, Cavanaugh, D, Gallup, JM, Pallares, FJ, Thacker, EL, Lager, KM, Meng, XJ & Halbur, PG 2004, 'Effect of porcine parvovirus vaccination on the development of PMWS in segregated early weaned pigs coinfected with type 2 porcine circovirus and porcine parvovirus', Veterinary Microbiology, vol. 98, no. 3-4, pp. 209-220. https://doi.org/10.1016/j.vetmic.2003.11.006

@article{f15084be071d47eaad9829c626a391f4,

title = "Effect of porcine parvovirus vaccination on the development of PMWS in segregated early weaned pigs coinfected with type 2 porcine circovirus and porcine parvovirus",

abstract = "The objectives of this study were to determine if coinfection of segregated early weaned (SEW) pigs with porcine circovirus type 2 (PCV2) and porcine parvovirus (PPV) induces an increase in the incidence of post-weaning multisystemic wasting syndrome (PMWS) compared to singular PCV2 infection, and to determine if vaccination against PPV protects pigs against PMWS associated with PCV2/PPV coinfection in SEW pigs. Seventy, 3-week-old, SEW pigs were randomly assigned to one of the five groups. Pigs in group 1 (n=14) served as the negative controls, group 2 pigs (n=14) were inoculated with PCV2, group 3 pigs (n=12) were inoculated with PPV, groups 4 (n=16) and 5 (n=14) pigs were inoculated with both PCV2 and PPV. Pigs in groups 1-3 and 5 were vaccinated with two doses of a killed parvovirus-leptospira-erysipelothrix (PLE) vaccine prior to inoculation. The PCV2/PPV-coinfected pigs (groups 4 and 5) had significantly (P<0.05) higher and more persistent fevers than the singular PCV2-infected pigs. One pig in each of the coinfected groups developed clinical disease (fever, respiratory disease, jaundice, weight loss) consistent with PMWS. Lymphoid depletion was significantly (P<0.05) more severe in the dually-infected pigs at 42 days post-inoculation (DPI). Vaccinated, coinfected pigs (group 5) remained viremic significantly (P<0.05) longer and had higher copy numbers of genomic PCV2 DNA in sera at 28, 35, and 42 DPI compared to the unvaccinated coinfected pigs (group 4). PPV-viremia was detected only in the unvaccinated group 4 pigs. PLE-vaccination prevented PPV-viremia but did not prevent clinical PMWS or reduce the severity of lymphoid depletion in PCV2/PPV-coinfected pigs. Evidence of increased incidence of clinical PMWS due to vaccination was not observed in this model.",

keywords = "Porcine circovirus type 2, Porcine parvovirus, Post-weaning multisystemic wasting syndrome, Vaccination",

author = "T. Opriessnig and M. Fenaux and S. Yu and Evans, {R. B.} and D. Cavanaugh and Gallup, {J. M.} and Pallares, {F. J.} and Thacker, {E. L.} and Lager, {K. M.} and Meng, {X. J.} and Halbur, {P. G.}",

note = "Funding Information: The authors thank Ann Vorwald for preparing the PPV-inoculum and for helpful advice with PPV serology. We also thank Pete Thomas and Marlin Hoogland for assistance with animal work. The study was funded by a grant from Pfizer Animal Health, Inc. ",

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doi = "10.1016/j.vetmic.2003.11.006",

language = "English (US)",

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T1 - Effect of porcine parvovirus vaccination on the development of PMWS in segregated early weaned pigs coinfected with type 2 porcine circovirus and porcine parvovirus

AU - Opriessnig, T.

AU - Fenaux, M.

AU - Yu, S.

AU - Evans, R. B.

AU - Cavanaugh, D.

AU - Gallup, J. M.

AU - Pallares, F. J.

AU - Thacker, E. L.

AU - Lager, K. M.

AU - Meng, X. J.

AU - Halbur, P. G.

N1 - Funding Information: The authors thank Ann Vorwald for preparing the PPV-inoculum and for helpful advice with PPV serology. We also thank Pete Thomas and Marlin Hoogland for assistance with animal work. The study was funded by a grant from Pfizer Animal Health, Inc.

PY - 2004/3/5

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N2 - The objectives of this study were to determine if coinfection of segregated early weaned (SEW) pigs with porcine circovirus type 2 (PCV2) and porcine parvovirus (PPV) induces an increase in the incidence of post-weaning multisystemic wasting syndrome (PMWS) compared to singular PCV2 infection, and to determine if vaccination against PPV protects pigs against PMWS associated with PCV2/PPV coinfection in SEW pigs. Seventy, 3-week-old, SEW pigs were randomly assigned to one of the five groups. Pigs in group 1 (n=14) served as the negative controls, group 2 pigs (n=14) were inoculated with PCV2, group 3 pigs (n=12) were inoculated with PPV, groups 4 (n=16) and 5 (n=14) pigs were inoculated with both PCV2 and PPV. Pigs in groups 1-3 and 5 were vaccinated with two doses of a killed parvovirus-leptospira-erysipelothrix (PLE) vaccine prior to inoculation. The PCV2/PPV-coinfected pigs (groups 4 and 5) had significantly (P<0.05) higher and more persistent fevers than the singular PCV2-infected pigs. One pig in each of the coinfected groups developed clinical disease (fever, respiratory disease, jaundice, weight loss) consistent with PMWS. Lymphoid depletion was significantly (P<0.05) more severe in the dually-infected pigs at 42 days post-inoculation (DPI). Vaccinated, coinfected pigs (group 5) remained viremic significantly (P<0.05) longer and had higher copy numbers of genomic PCV2 DNA in sera at 28, 35, and 42 DPI compared to the unvaccinated coinfected pigs (group 4). PPV-viremia was detected only in the unvaccinated group 4 pigs. PLE-vaccination prevented PPV-viremia but did not prevent clinical PMWS or reduce the severity of lymphoid depletion in PCV2/PPV-coinfected pigs. Evidence of increased incidence of clinical PMWS due to vaccination was not observed in this model.

AB - The objectives of this study were to determine if coinfection of segregated early weaned (SEW) pigs with porcine circovirus type 2 (PCV2) and porcine parvovirus (PPV) induces an increase in the incidence of post-weaning multisystemic wasting syndrome (PMWS) compared to singular PCV2 infection, and to determine if vaccination against PPV protects pigs against PMWS associated with PCV2/PPV coinfection in SEW pigs. Seventy, 3-week-old, SEW pigs were randomly assigned to one of the five groups. Pigs in group 1 (n=14) served as the negative controls, group 2 pigs (n=14) were inoculated with PCV2, group 3 pigs (n=12) were inoculated with PPV, groups 4 (n=16) and 5 (n=14) pigs were inoculated with both PCV2 and PPV. Pigs in groups 1-3 and 5 were vaccinated with two doses of a killed parvovirus-leptospira-erysipelothrix (PLE) vaccine prior to inoculation. The PCV2/PPV-coinfected pigs (groups 4 and 5) had significantly (P<0.05) higher and more persistent fevers than the singular PCV2-infected pigs. One pig in each of the coinfected groups developed clinical disease (fever, respiratory disease, jaundice, weight loss) consistent with PMWS. Lymphoid depletion was significantly (P<0.05) more severe in the dually-infected pigs at 42 days post-inoculation (DPI). Vaccinated, coinfected pigs (group 5) remained viremic significantly (P<0.05) longer and had higher copy numbers of genomic PCV2 DNA in sera at 28, 35, and 42 DPI compared to the unvaccinated coinfected pigs (group 4). PPV-viremia was detected only in the unvaccinated group 4 pigs. PLE-vaccination prevented PPV-viremia but did not prevent clinical PMWS or reduce the severity of lymphoid depletion in PCV2/PPV-coinfected pigs. Evidence of increased incidence of clinical PMWS due to vaccination was not observed in this model.

KW - Porcine circovirus type 2

KW - Porcine parvovirus

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KW - Vaccination

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Effect of porcine parvovirus vaccination on the development of PMWS in segregated early weaned pigs coinfected with type 2 porcine circovirus and porcine parvovirus

Abstract

Bibliographical note

Keywords

UN SDGs

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