Effect of pH and sulfate concentration on hydrogen production using anaerobic mixed microflora

Jae Hoon Hwang; Jeong A. Choi; R. A I Abou-Shanab; Amit Bhatnagar; Booki Min; Hocheol Song; Eva Kumar; Jaeyoung Choi; Eung Seok Lee; Yong Je Kim; Sukkee Um; Dae Sung Lee; Byong Hun Jeon

doi:10.1016/j.ijhydene.2009.10.022

Effect of pH and sulfate concentration on hydrogen production using anaerobic mixed microflora

Jae Hoon Hwang, Jeong A. Choi, R. A I Abou-Shanab, Amit Bhatnagar, Booki Min, Hocheol Song, Eva Kumar, Jaeyoung Choi, Eung Seok Lee, Yong Je Kim, Sukkee Um, Dae Sung Lee, Byong Hun Jeon

Biotechnology Institute

Research output: Contribution to journal › Article › peer-review

68 Scopus citations

Abstract

The effects of varying sulfate concentrations with pH on continuous fermentative hydrogen production were studied using anaerobic mixed cultures growing on a glucose substrate in a chemostat reactor. The maximum hydrogen production rate was 2.8 L/day at pH 5.5 and sulfate concentration of 3000 mg/L. Hydrogen production and residual sulfate level decreased with increasing the pH from 5.5 to 6.2. The volatile fatty acids (VFAs) and ethanol fractions in the effluent were in the order of butyric acid (HBu) > acetic acid (HAc) > ethanol > propionic acid (HPr). Fluorescence In Situ Hybridization (FISH) analysis revealed the presence of hydrogen producing bacteria (HPB) under all pH ranges while sulfate reducing bacteria (SRB) were present at pH 5.8 and 6.2. The inhibition in hydrogen production by SRB at pH 6.2 diminished entirely by lowering to pH 5.5, at which activity of SRB is substantially suppressed.

Original language	English (US)
Pages (from-to)	9702-9710
Number of pages	9
Journal	International Journal of Hydrogen Energy
Volume	34
Issue number	24
DOIs	https://doi.org/10.1016/j.ijhydene.2009.10.022
State	Published - Dec 2009

Bibliographical note

Funding Information:
This work was supported by 21st Frontier research project (Sustainable Water Resources Research Center 3-4-3), Global Research Laboratory project (Korea Institute of Geosciences and Mineral Resources NP2008-019), Basic Research Project of the Korea Institute of Geoscience & Mineral Resources (KIGAM) funded by the Ministry of Science and Technology of Korea and Brain Korea-21 (BK-21) program of the Ministry of Education.

Keywords

Anaerobic mixed microflora
Fluorescence in situ hybridization
Hydrogen production
Sulfate
pH

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.ijhydene.2009.10.022

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Hwang, J. H., Choi, J. A., Abou-Shanab, R. A. I., Bhatnagar, A., Min, B., Song, H., Kumar, E., Choi, J., Lee, E. S., Kim, Y. J., Um, S., Lee, D. S., & Jeon, B. H. (2009). Effect of pH and sulfate concentration on hydrogen production using anaerobic mixed microflora. International Journal of Hydrogen Energy, 34(24), 9702-9710. https://doi.org/10.1016/j.ijhydene.2009.10.022

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title = "Effect of pH and sulfate concentration on hydrogen production using anaerobic mixed microflora",

abstract = "The effects of varying sulfate concentrations with pH on continuous fermentative hydrogen production were studied using anaerobic mixed cultures growing on a glucose substrate in a chemostat reactor. The maximum hydrogen production rate was 2.8 L/day at pH 5.5 and sulfate concentration of 3000 mg/L. Hydrogen production and residual sulfate level decreased with increasing the pH from 5.5 to 6.2. The volatile fatty acids (VFAs) and ethanol fractions in the effluent were in the order of butyric acid (HBu) > acetic acid (HAc) > ethanol > propionic acid (HPr). Fluorescence In Situ Hybridization (FISH) analysis revealed the presence of hydrogen producing bacteria (HPB) under all pH ranges while sulfate reducing bacteria (SRB) were present at pH 5.8 and 6.2. The inhibition in hydrogen production by SRB at pH 6.2 diminished entirely by lowering to pH 5.5, at which activity of SRB is substantially suppressed.",

keywords = "Anaerobic mixed microflora, Fluorescence in situ hybridization, Hydrogen production, Sulfate, pH",

author = "Hwang, {Jae Hoon} and Choi, {Jeong A.} and Abou-Shanab, {R. A I} and Amit Bhatnagar and Booki Min and Hocheol Song and Eva Kumar and Jaeyoung Choi and Lee, {Eung Seok} and Kim, {Yong Je} and Sukkee Um and Lee, {Dae Sung} and Jeon, {Byong Hun}",

note = "Funding Information: This work was supported by 21st Frontier research project (Sustainable Water Resources Research Center 3-4-3), Global Research Laboratory project (Korea Institute of Geosciences and Mineral Resources NP2008-019), Basic Research Project of the Korea Institute of Geoscience & Mineral Resources (KIGAM) funded by the Ministry of Science and Technology of Korea and Brain Korea-21 (BK-21) program of the Ministry of Education.",

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AU - Hwang, Jae Hoon

AU - Choi, Jeong A.

AU - Abou-Shanab, R. A I

AU - Bhatnagar, Amit

AU - Min, Booki

AU - Song, Hocheol

AU - Kumar, Eva

AU - Choi, Jaeyoung

AU - Lee, Eung Seok

AU - Kim, Yong Je

AU - Um, Sukkee

AU - Lee, Dae Sung

AU - Jeon, Byong Hun

N1 - Funding Information: This work was supported by 21st Frontier research project (Sustainable Water Resources Research Center 3-4-3), Global Research Laboratory project (Korea Institute of Geosciences and Mineral Resources NP2008-019), Basic Research Project of the Korea Institute of Geoscience & Mineral Resources (KIGAM) funded by the Ministry of Science and Technology of Korea and Brain Korea-21 (BK-21) program of the Ministry of Education.

PY - 2009/12

Y1 - 2009/12

N2 - The effects of varying sulfate concentrations with pH on continuous fermentative hydrogen production were studied using anaerobic mixed cultures growing on a glucose substrate in a chemostat reactor. The maximum hydrogen production rate was 2.8 L/day at pH 5.5 and sulfate concentration of 3000 mg/L. Hydrogen production and residual sulfate level decreased with increasing the pH from 5.5 to 6.2. The volatile fatty acids (VFAs) and ethanol fractions in the effluent were in the order of butyric acid (HBu) > acetic acid (HAc) > ethanol > propionic acid (HPr). Fluorescence In Situ Hybridization (FISH) analysis revealed the presence of hydrogen producing bacteria (HPB) under all pH ranges while sulfate reducing bacteria (SRB) were present at pH 5.8 and 6.2. The inhibition in hydrogen production by SRB at pH 6.2 diminished entirely by lowering to pH 5.5, at which activity of SRB is substantially suppressed.

AB - The effects of varying sulfate concentrations with pH on continuous fermentative hydrogen production were studied using anaerobic mixed cultures growing on a glucose substrate in a chemostat reactor. The maximum hydrogen production rate was 2.8 L/day at pH 5.5 and sulfate concentration of 3000 mg/L. Hydrogen production and residual sulfate level decreased with increasing the pH from 5.5 to 6.2. The volatile fatty acids (VFAs) and ethanol fractions in the effluent were in the order of butyric acid (HBu) > acetic acid (HAc) > ethanol > propionic acid (HPr). Fluorescence In Situ Hybridization (FISH) analysis revealed the presence of hydrogen producing bacteria (HPB) under all pH ranges while sulfate reducing bacteria (SRB) were present at pH 5.8 and 6.2. The inhibition in hydrogen production by SRB at pH 6.2 diminished entirely by lowering to pH 5.5, at which activity of SRB is substantially suppressed.

KW - Anaerobic mixed microflora

KW - Fluorescence in situ hybridization

KW - Hydrogen production

KW - Sulfate

KW - pH

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JO - International Journal of Hydrogen Energy

JF - International Journal of Hydrogen Energy

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ER -

Effect of pH and sulfate concentration on hydrogen production using anaerobic mixed microflora

Abstract

Bibliographical note

Keywords

UN SDGs

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