Effect of paratuberculosis on the diagnosis of bovine tuberculosis in a cattle herd with a mixed infection using interferon-gamma detection assay

Julio Álvarez; Lucía de Juan; Javier Bezos; Beatriz Romero; Jose Luis Sáez; Sergio Marqués; Concepción Domínguez; Olga Mínguez; Baudilio Fernández-Mardomingo; Ana Mateos; Lucas Domínguez; Alicia Aranaz

doi:10.1016/j.vetmic.2008.09.060

Effect of paratuberculosis on the diagnosis of bovine tuberculosis in a cattle herd with a mixed infection using interferon-gamma detection assay

Julio Álvarez, Lucía de Juan, Javier Bezos, Beatriz Romero, Jose Luis Sáez, Sergio Marqués, Concepción Domínguez, Olga Mínguez, Baudilio Fernández-Mardomingo, Ana Mateos, Lucas Domínguez, Alicia Aranaz

Research output: Contribution to journal › Article › peer-review

71 Scopus citations

Abstract

Interferon-gamma (IFN-γ) detection assay is being applied as an ancillary test to tuberculin tests in the diagnosis of bovine tuberculosis to detect the maximum number of infected animals. Among possible factors influencing the performance of tuberculosis-diagnostic tests, paratuberculosis, a widespread disease in Spain and other European countries, has been pointed out as a cause of false positive reactions. Still, its effect on the sensitivity of these tests in cattle has yet to be fully characterized. The impact of paratuberculosis in the apparent sensitivity of IFN-γ assay was studied in a bullfighting cattle herd with a mixed tuberculosis-paratuberculosis infection, using culture of Mycobacterium bovis and Mycobacterium avium paratuberculosis as the gold standard to determine the infection status of every animal. A total of 218 animals were slaughtered and sampled for bacteriology after blood sampling. IFN-γ assay showed a lower apparent sensitivity in animals with a mixed infection (50%) compared to all animals suffering tuberculosis (78.3%). This finding indicates that the presence of paratuberculosis in tuberculosis-infected herds could imply a serious impairment in the sensitivity of IFN-γ detection test.

Original language	English (US)
Pages (from-to)	389-393
Number of pages	5
Journal	Veterinary Microbiology
Volume	135
Issue number	3-4
DOIs	https://doi.org/10.1016/j.vetmic.2008.09.060
State	Published - Mar 30 2009
Externally published	Yes

Bibliographical note

Funding Information:
This research was funded by project AGL2006-06206 of the Spanish Ministry of Science and Technology, ParaTBTools (STREP 23106) of the European Union, and the Spanish Ministry of Agriculture, Food and Fisheries. J. Álvarez was the recipient of a predoctoral grant assigned by the Spanish Ministry of Education and Culture. The group is a partner of the coordination action “Veterinary European Network on Mycobacteria (VENoMYC)” funded by the European Union.

Keywords

Cattle
Diagnosis
IFN-γ
Paratuberculosis
Tuberculosis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.vetmic.2008.09.060

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Álvarez, J., de Juan, L., Bezos, J., Romero, B., Sáez, J. L., Marqués, S., Domínguez, C., Mínguez, O., Fernández-Mardomingo, B., Mateos, A., Domínguez, L., & Aranaz, A. (2009). Effect of paratuberculosis on the diagnosis of bovine tuberculosis in a cattle herd with a mixed infection using interferon-gamma detection assay. Veterinary Microbiology, 135(3-4), 389-393. https://doi.org/10.1016/j.vetmic.2008.09.060

Álvarez, J, de Juan, L, Bezos, J, Romero, B, Sáez, JL, Marqués, S, Domínguez, C, Mínguez, O, Fernández-Mardomingo, B, Mateos, A, Domínguez, L & Aranaz, A 2009, 'Effect of paratuberculosis on the diagnosis of bovine tuberculosis in a cattle herd with a mixed infection using interferon-gamma detection assay', Veterinary Microbiology, vol. 135, no. 3-4, pp. 389-393. https://doi.org/10.1016/j.vetmic.2008.09.060

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abstract = "Interferon-gamma (IFN-γ) detection assay is being applied as an ancillary test to tuberculin tests in the diagnosis of bovine tuberculosis to detect the maximum number of infected animals. Among possible factors influencing the performance of tuberculosis-diagnostic tests, paratuberculosis, a widespread disease in Spain and other European countries, has been pointed out as a cause of false positive reactions. Still, its effect on the sensitivity of these tests in cattle has yet to be fully characterized. The impact of paratuberculosis in the apparent sensitivity of IFN-γ assay was studied in a bullfighting cattle herd with a mixed tuberculosis-paratuberculosis infection, using culture of Mycobacterium bovis and Mycobacterium avium paratuberculosis as the gold standard to determine the infection status of every animal. A total of 218 animals were slaughtered and sampled for bacteriology after blood sampling. IFN-γ assay showed a lower apparent sensitivity in animals with a mixed infection (50%) compared to all animals suffering tuberculosis (78.3%). This finding indicates that the presence of paratuberculosis in tuberculosis-infected herds could imply a serious impairment in the sensitivity of IFN-γ detection test.",

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AU - Sáez, Jose Luis

AU - Marqués, Sergio

AU - Domínguez, Concepción

AU - Mínguez, Olga

AU - Fernández-Mardomingo, Baudilio

AU - Mateos, Ana

AU - Domínguez, Lucas

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Effect of paratuberculosis on the diagnosis of bovine tuberculosis in a cattle herd with a mixed infection using interferon-gamma detection assay

Abstract

Bibliographical note

Keywords

UN SDGs

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