Effect of microgravity on antioxidant defense and oxidative stress in rat liver

J. Hollander, M. Gore, R. Fiebig, D. Powers, M. Hulling, S. Legichtweis, C. Leeuwenburgh, L. L. Ji

Research output: Contribution to journalArticlepeer-review

Abstract

The influence of microgravity on liver oxidative stress and antioxidant defenses were investigated in 3 groups of Sprague-Dawley rats: flight (F). aboard Space Shuttle flight 63 for 8 days; flight-simulated module (A); and control (C). Animals were injected with 1 mg interleukin-2 (II,-2) /kg body wt or saline (S) 24 h before lift-off. Liver wts in F and A were greater than that in C (P<0.01 ). Liver protein concentration decreased in F vs A (P<0.05). Activities of glutathione (GSH) peroxidase (GPX) and GSH Stransferase were decreased in F and A vs C (P<0.01 ). Catalase (CAT) activity was decreased in F vs A and C (P<0.05). Activities of glutathione reductase and Superoxide dismutase (Cu-Zn SOD and Mn SOD) were unchanged. The relative abundance of mRNA for GPX. CAT. Cu-Zn SOD, and Mn SOD was reduced in F vs A (P<0.05). Cu-Zn SOD and Mn SOD protein contents were lower (P<0.05) in A vs C and F. Concentrations of total glutathione, GSH, and glutathione disulfide decreased with F vs A and C (P<0.01). y-Glutamylcysteine synthase activity was not altered with treatments. γ-Glutamyl transpeptidase activity was increased in F with IL2, but not with S (P<0.01). Lipid peroxidation was greater in F vs A and C (PO.05), whereas IL-2 attenuated this effect in F (P<0.05). These results suggest that microgravity is associated with increased oxidative stress and may down-regulate hepatic antioxidant enzyme activity. mRNA abundance, and GSH content.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number3
StatePublished - Dec 1 1996

Fingerprint Dive into the research topics of 'Effect of microgravity on antioxidant defense and oxidative stress in rat liver'. Together they form a unique fingerprint.

Cite this