Effect of magnesium supplementation on circulating biomarkers of cardiovascular disease

Alvaro Alonso, Lin Y. Chen, Kyle D. Rudser, Faye L. Norby, Mary R. Rooney, Pamela L. Lutsey

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


(1) Background: Magnesium supplementation may be effective for the prevention of cardiometabolic diseases, but the mechanisms are unclear. Proteomic approaches can assist in identifying the underlying mechanisms. (2) Methods: We collected repeated blood samples from 52 individuals enrolled in a double-blind trial which randomized participants 1:1 to oral magnesium supplementation (400 mg magnesium/day in the form of magnesium oxide) or a matching placebo for 10 weeks. Plasma levels of 91 proteins were measured at baseline with follow-up samples using the Olink Cardiovascular Disease III proximity extension assay panel and were modeled as arbitrary units in a log2 scale. We evaluated the effect of oral magnesium supplementation for changes in protein levels and the baseline association between serum magnesium and protein levels. The Holm procedure was used to adjust for multiple comparisons. (3) Results: Participants were 73% women, 94% white, and had a mean age of 62. Changes in proteins did not significantly differ between the two intervention groups after correction for multiple comparisons. The most statistically significant effects were on myoglobin [difference −0.319 log2 units, 95% confidence interval (CI) (−0.550, −0.088), p = 0.008], tartrate-resistant acid phosphatase type 5 (−0.187, (−0.328, −0.045), p = 0.011), tumor necrosis factor ligand superfamily member 13B (−0.181, (−0.332, −0.031), p = 0.019), ST2 protein (−0.198, (−0.363, −0.032), p = 0.020), and interleukin-1 receptor type 1 (−0.144, (−0.273, −0.015), p = 0.029). Similarly, none of the associations of baseline serum magnesium with protein levels were significant after correction for multiple comparisons. (4) Conclusions: Although we did not identify statistically significant effects of oral magnesium supplementation in this relatively small study, this study demonstrates the value of proteomic approaches for the investigation of mechanisms underlying the beneficial effects of magnesium supplementation. Clinical Trials Registration: ClinicalTrials.gov NCT02837328.

Original languageEnglish (US)
Article number1697
Issue number6
StatePublished - Jun 2020

Bibliographical note

Funding Information:
Funding: This work was supported by internal funds of the Division of Epidemiology and Community Health, University of Minnesota, the McKnight Land-Grant Professorship funds, the American Heart Association grant 16EIA26410001 (Alonso), the National Heart, Lung, And Blood Institute grants T32HL007779 and T32HL007024 (Rooney), the National Center for Advancing Translational Sciences award UL1TR002494. Dr. Alonso was additionally supported by the National Heart, Lung, And Blood Institute of the National Institutes of Health under award number K24HL148521. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.


  • Magnesium
  • Proteomics
  • Randomized trial

PubMed: MeSH publication types

  • Journal Article
  • Randomized Controlled Trial


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