Effect of lipoxygenase inhibition on mucous glycoprotein secretion from chinchilla middle ear epithelial cells in vitro

Youngki Kim, Frank Ondrey, Jizhen Lin, Chris Lees, Steven K. Juhn

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Lipoxygenase is an enzyme that metabolizes arachidonic acid down to leukotrienes. Recent studies have shown that the enzyme is implicated in mucous glycoprotein (MGP) secretion stimulated by inflammatory mediators in the airways, suggesting its possible role in secretion of MGP from middle ear epithelial cells. To investigate a correlation between MGP secretion and the arachidonic acid metabolites, we examined the effects of nordihydroguaretic acid (NDGA, both a cyclooxygenase and lipoxygenase inhibitor), low-dose indomethacin (an inhibitor of cyclooxygenase), and A63162 (an inhibitor of lipoxygenase) on MGP secretion in cultured chinchilla middle ear epithelial cells. It was found that lipoxygenase inhibition led to reduction of MGP secretion from cultured chinchilla middle ear epithelial cells, while cyclooxygenase inhibition did not. Both cyclooxygenase and lipoxygenase inhibition resulted in profound blockage of MGP secretion in baseline and platelet activating factor-stimulated MGP secretion. It was concluded, therefore, that MGP secretion was linked to arachidonic acid metabolites, especially lipoxygenase products.

Original languageEnglish (US)
Pages (from-to)916-921
Number of pages6
JournalAnnals of Otology, Rhinology and Laryngology
Volume105
Issue number11
DOIs
StatePublished - 1996

Keywords

  • lipoxygenase
  • middle ear epithelial cells in vitro
  • mucous glycoprotein secretion
  • otitis media with effusion
  • platelet activating factor

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