Effect of lead on Sertoli-germ cell coculture of rat

Neeta Adhikari, Neelima Sinha, D. K. Saxena

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29 Scopus citations

Abstract

Mixed cultures of Sertoli and germ cells were prepared from rat testes and their response to lead (Pb) was studied. Cultures consisted of a monolayer of Sertoli cells to which clusters of germ cells were attached. The effect of Pb added as lead acetate was tested at 0.0, 0.4, 4.0 and 40.0 μM for 24 and 48 h intervals. Addition of Pb to the culture medium caused germ cells to progressively detach from the Sertoli cell monolayer into the medium in a concentration and duration dependent manner Viability of the detached cells as judged by trypan blue exclusion test showed a decrease with increase in time and concentration of Pb. Significant leakage of lactate dehydrogenase (LDH) was recorded in the culture media only at the higher concentrations of 4.0 and 40.0 μM. Thus Pb at the doses tested induced cytotoxicity in rat Sertoli-germ cell coculture. Copyright (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish (US)
Pages (from-to)45-49
Number of pages5
JournalToxicology Letters
Volume116
Issue number1-2
DOIs
StatePublished - Jul 27 2000
Externally publishedYes

Bibliographical note

Funding Information:
We thank the Director of Industrial Toxicology. Research Center for his keen interest and encouragement in this study. The authors, NA and NS are thankful to Council of Scientific and Industrial Research, New Delhi, for providing financial assistance as Senior Research Associateships. The help rendered by Dr Neeraj Mathur for statistical analysis is duly acknowledged.

Keywords

  • Lead
  • Rat
  • Sertoli-germ cell coculture

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