Effect of kosmotropicity of ionic liquids on the enzyme stability in aqueous solutions

Hua Zhao, Olarongbe Olubajo, Zhiyan Song, Artez L. Sims, Terra E. Person, Rasheed A. Lawal, La Dena A. Holley

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177 Scopus citations


This paper examined the effect of several pyridinium and imidazolium-based ionic liquids (ILs) on the protease stability in aqueous solutions. In general, the enzyme was found quite active at low concentrations of hydrophilic ILs. In aqueous environment, the enzyme was stabilized by the kosmotropic anions (such as CF3COO- and CH3COO-) and chaotropic cations (such as [BuPy]+ and [EMIM]+), but was destabilized by chaotropic anions (such as tosylate and BF4 -) and kosmotropic cations (such as [BMIM]+).

Original languageEnglish (US)
Pages (from-to)15-25
Number of pages11
JournalBioorganic Chemistry
Issue number1
StatePublished - Feb 2006
Externally publishedYes

Bibliographical note

Funding Information:
This project was supported by the NIH-EARDA grants (No. 5 G11 HD 32861-07 and No. 5 G11 HD 32861-08). Partial financial support was provided by a NIH-MBRS grant. We are also indebted to Amano Enzyme USA for providing samples of enzymes.


  • Activity
  • Chaotrope
  • Enzyme
  • Ionic liquid
  • Kosmotrope
  • Kosmotropicity
  • Stability


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