Effect of inositol hexaphosphate-loaded red blood cells (RBCs) on the rheology of sickle RBCs

Yann Lamarre, Vanessa Bourgeaux, Aurélien Pichon, Max R. Hardeman, Yannick Campion, Marise Hardeman-Zijp, Cyril Martin, Jean Paul Richalet, Françoise Bernaudin, Françoise Driss, Yann Godfrin, Philippe Connes

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Background: The recent in vitro demonstration that inositol hexaphosphate-loaded red blood cells (IHP-RBCs) may reduce the risks of sickling of sickle RBCs (SS RBCs) exposed to hypoxia make these modified RBCs potentially useful in transfused sickle cell anemia (SCA) patients. Study Desing and Methods: Hemorheologic properties of IHP-RBCs, normal RBCs (AA RBCs), SS RBCs, SS RBCs plus AA RBCs, and SS RBCs plus IHP-RBCs were compared under normoxia and/or after hypoxic challenges. Results: Although IHP-RBCs have reduced deformability compared with SS RBCs or AA RBCs, IHP-RBCs exhibited lower aggregability than AA RBCs and SS RBCs and, when mixed with SS RBCs, the aggregation level was below the one of SS RBCs alone or SS RBCs plus AA RBCs. Blood viscosity of SS RBC plus IHP-RBC suspension was lower than the viscosity of SS RBCs alone and greater than viscosity of SS RBCs plus AA RBCs. The hypoxic challenge was detrimental for deformability and viscosity of SS RBCs alone or SS plus AA RBC suspension but not for SS plus IHP-RBC suspension. Conclusion: Our results support the fact that IHP-RBCs could be useful in SCA by decreasing RBC aggregation and blunting the adverse effects of hypoxia on RBC deformability and blood viscosity.

Original languageEnglish (US)
Pages (from-to)627-636
Number of pages10
JournalTransfusion
Volume53
Issue number3
DOIs
StatePublished - Mar 2013

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