Effect of Housing Types on Growth, Feeding, Physical Activity, and Anxiety-Like Behavior in Male Sprague-Dawley Rats

Jennifer A. Teske; Claudio Esteban Perez-Leighton; Emily E. Noble; Chuanfeng Wang; Charles J. Billington; Catherine M. Kotz

doi:10.3389/fnut.2016.00004

Effect of Housing Types on Growth, Feeding, Physical Activity, and Anxiety-Like Behavior in Male Sprague-Dawley Rats

Jennifer A. Teske, Claudio Esteban Perez-Leighton, Emily E. Noble, Chuanfeng Wang, Charles J. Billington, Catherine M. Kotz

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Background: Animal welfare and accurate data collection are equally important in rodent research. Housing influences study outcomes and can challenge studies that monitor feeding, so housing choice needs to be evidence-based. The goal of these studies was to (1) compare established measures of well-being between rodents housed in wire grid-bottom floors with a resting platform compared to solid-bottom floors with bedding and (2) determine whether presence of a chewable device (Nylabone) affects orexin-A-induced hyperphagia. Methods: Rodents were crossed over to the alternate housing twice after 2-week periods. Time required to complete food intake measurements was recorded as an indicator of feasibility. Food intake stimulated by orexin-A was compared with and without the Nylabone. Blood corticosterone and hypothalamic BDNF were assessed. Results: Housing had no effect on growth, energy expenditure, corticosterone, hypothalamic BDNF, behavior, and anxiety measures. Food intake was disrupted after housing cross-over. Time required to complete food intake measurements was significantly higher for solid-bottom bedded cages. The Nylabone had no effect on orexin-A-stimulated feeding. Conclusion: Well-being is not significantly different between rodents housed on grid-bottom floors and those in solid-bottom-bedded cages based on overall growth and feeding but alternating between housing confounds measures of feeding.

Original language	English (US)
Article number	4
Journal	Frontiers in Nutrition
Volume	3
DOIs	https://doi.org/10.3389/fnut.2016.00004
State	Published - Feb 4 2016

Bibliographical note

Funding Information:
Funding for this research and publication was supported in part by the Minnesota Partnership for Biotechnology and Genomics [1I01RX000441-01A2 (MPI) to CK], a Career Development Award-level 2 from the United States Department of Veterans Affairs Rehabilitation Research and Development Service (F7212W to JT), the United States Department of Agriculture (ARZT-1360220-H23-150 to JT), the National Institutes of Health (NIDDK R01-DK078985 to CK, 2P30-DK050456 to CB, and 1R01-DK080782 to CW), and CONICYT, Gobierno de Chile, Programa FONDECYT Regular (1150274 to CPL). Funding agencies did not have a role in the study design, data collection, analysis, decision to publish, or preparation of the manuscript.

Publisher Copyright:
© Copyright © 2016 Teske, Perez-Leighton, Noble, Wang, Billington and Kotz.

Keywords

anxiety
cognition
environmental enrichment
microenvironment
stress

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10.3389/fnut.2016.00004

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@article{9199b0b341c142a8affa9ba61c4c83de,

title = "Effect of Housing Types on Growth, Feeding, Physical Activity, and Anxiety-Like Behavior in Male Sprague-Dawley Rats",

abstract = "Background: Animal welfare and accurate data collection are equally important in rodent research. Housing influences study outcomes and can challenge studies that monitor feeding, so housing choice needs to be evidence-based. The goal of these studies was to (1) compare established measures of well-being between rodents housed in wire grid-bottom floors with a resting platform compared to solid-bottom floors with bedding and (2) determine whether presence of a chewable device (Nylabone) affects orexin-A-induced hyperphagia. Methods: Rodents were crossed over to the alternate housing twice after 2-week periods. Time required to complete food intake measurements was recorded as an indicator of feasibility. Food intake stimulated by orexin-A was compared with and without the Nylabone. Blood corticosterone and hypothalamic BDNF were assessed. Results: Housing had no effect on growth, energy expenditure, corticosterone, hypothalamic BDNF, behavior, and anxiety measures. Food intake was disrupted after housing cross-over. Time required to complete food intake measurements was significantly higher for solid-bottom bedded cages. The Nylabone had no effect on orexin-A-stimulated feeding. Conclusion: Well-being is not significantly different between rodents housed on grid-bottom floors and those in solid-bottom-bedded cages based on overall growth and feeding but alternating between housing confounds measures of feeding.",

keywords = "anxiety, cognition, environmental enrichment, microenvironment, stress",

author = "Teske, {Jennifer A.} and Perez-Leighton, {Claudio Esteban} and Noble, {Emily E.} and Chuanfeng Wang and Billington, {Charles J.} and Kotz, {Catherine M.}",

note = "Funding Information: Funding for this research and publication was supported in part by the Minnesota Partnership for Biotechnology and Genomics [1I01RX000441-01A2 (MPI) to CK], a Career Development Award-level 2 from the United States Department of Veterans Affairs Rehabilitation Research and Development Service (F7212W to JT), the United States Department of Agriculture (ARZT-1360220-H23-150 to JT), the National Institutes of Health (NIDDK R01-DK078985 to CK, 2P30-DK050456 to CB, and 1R01-DK080782 to CW), and CONICYT, Gobierno de Chile, Programa FONDECYT Regular (1150274 to CPL). Funding agencies did not have a role in the study design, data collection, analysis, decision to publish, or preparation of the manuscript. Publisher Copyright: {\textcopyright} Copyright {\textcopyright} 2016 Teske, Perez-Leighton, Noble, Wang, Billington and Kotz.",

year = "2016",

month = feb,

day = "4",

doi = "10.3389/fnut.2016.00004",

language = "English (US)",

volume = "3",

journal = "Frontiers in Nutrition",

issn = "2296-861X",

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TY - JOUR

T1 - Effect of Housing Types on Growth, Feeding, Physical Activity, and Anxiety-Like Behavior in Male Sprague-Dawley Rats

AU - Teske, Jennifer A.

AU - Perez-Leighton, Claudio Esteban

AU - Noble, Emily E.

AU - Wang, Chuanfeng

AU - Billington, Charles J.

AU - Kotz, Catherine M.

N1 - Funding Information: Funding for this research and publication was supported in part by the Minnesota Partnership for Biotechnology and Genomics [1I01RX000441-01A2 (MPI) to CK], a Career Development Award-level 2 from the United States Department of Veterans Affairs Rehabilitation Research and Development Service (F7212W to JT), the United States Department of Agriculture (ARZT-1360220-H23-150 to JT), the National Institutes of Health (NIDDK R01-DK078985 to CK, 2P30-DK050456 to CB, and 1R01-DK080782 to CW), and CONICYT, Gobierno de Chile, Programa FONDECYT Regular (1150274 to CPL). Funding agencies did not have a role in the study design, data collection, analysis, decision to publish, or preparation of the manuscript. Publisher Copyright: © Copyright © 2016 Teske, Perez-Leighton, Noble, Wang, Billington and Kotz.

PY - 2016/2/4

Y1 - 2016/2/4

N2 - Background: Animal welfare and accurate data collection are equally important in rodent research. Housing influences study outcomes and can challenge studies that monitor feeding, so housing choice needs to be evidence-based. The goal of these studies was to (1) compare established measures of well-being between rodents housed in wire grid-bottom floors with a resting platform compared to solid-bottom floors with bedding and (2) determine whether presence of a chewable device (Nylabone) affects orexin-A-induced hyperphagia. Methods: Rodents were crossed over to the alternate housing twice after 2-week periods. Time required to complete food intake measurements was recorded as an indicator of feasibility. Food intake stimulated by orexin-A was compared with and without the Nylabone. Blood corticosterone and hypothalamic BDNF were assessed. Results: Housing had no effect on growth, energy expenditure, corticosterone, hypothalamic BDNF, behavior, and anxiety measures. Food intake was disrupted after housing cross-over. Time required to complete food intake measurements was significantly higher for solid-bottom bedded cages. The Nylabone had no effect on orexin-A-stimulated feeding. Conclusion: Well-being is not significantly different between rodents housed on grid-bottom floors and those in solid-bottom-bedded cages based on overall growth and feeding but alternating between housing confounds measures of feeding.

AB - Background: Animal welfare and accurate data collection are equally important in rodent research. Housing influences study outcomes and can challenge studies that monitor feeding, so housing choice needs to be evidence-based. The goal of these studies was to (1) compare established measures of well-being between rodents housed in wire grid-bottom floors with a resting platform compared to solid-bottom floors with bedding and (2) determine whether presence of a chewable device (Nylabone) affects orexin-A-induced hyperphagia. Methods: Rodents were crossed over to the alternate housing twice after 2-week periods. Time required to complete food intake measurements was recorded as an indicator of feasibility. Food intake stimulated by orexin-A was compared with and without the Nylabone. Blood corticosterone and hypothalamic BDNF were assessed. Results: Housing had no effect on growth, energy expenditure, corticosterone, hypothalamic BDNF, behavior, and anxiety measures. Food intake was disrupted after housing cross-over. Time required to complete food intake measurements was significantly higher for solid-bottom bedded cages. The Nylabone had no effect on orexin-A-stimulated feeding. Conclusion: Well-being is not significantly different between rodents housed on grid-bottom floors and those in solid-bottom-bedded cages based on overall growth and feeding but alternating between housing confounds measures of feeding.

KW - anxiety

KW - cognition

KW - environmental enrichment

KW - microenvironment

KW - stress

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DO - 10.3389/fnut.2016.00004

M3 - Article

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SN - 2296-861X

VL - 3

JO - Frontiers in Nutrition

JF - Frontiers in Nutrition

M1 - 4

ER -

Effect of Housing Types on Growth, Feeding, Physical Activity, and Anxiety-Like Behavior in Male Sprague-Dawley Rats

Abstract

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