Effect of harvesting protocol on performance of a hollow fiber bioreactor

Michael J. Gramer, Douglas M. Poeschl, Mark J. Conroy, Bruce E. Hammer

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

In this study, a bioreactor subject to Starling flow in closed shell batch harvest mode was compared to two forms of additional forced extracapillary (EC) space convection including EC circulation and EC cycling. Despite the presence of Starling flow as the dominant EC convection mechanism in the batch harvest system, the bioreactor start up was fairly good. However, the antibody productivity of the batch harvest system fell off rapidly after day 20 resulting in only 4.5 g of antibody produced. EC circulation with flow parallel to the fibers had a slightly better start up than the batch harvest. However, the antibody productivity also dropped after day 20 with EC circulation, resulting in only 7.5 g of antibody produced. EC cycling with flow both parallel and perpendicular to the fibers resulted in a start up similar to that of EC circulation. However, in contrast to the other two systems, antibody productivity in the EC cycling system was stable over the 60-day experiment resulting in the production of 23 g of antibody. These results demonstrate the importance of inducing the proper flow distribution in the EC space to allow consistent and stable production in hollow fiber bioreactors.

Original languageEnglish (US)
Pages (from-to)334-340
Number of pages7
JournalBiotechnology and bioengineering
Volume65
Issue number3
DOIs
StatePublished - Nov 5 1999

Keywords

  • Antibody production
  • Convection
  • Cycling
  • Hollow fiber bioreactor
  • Hybridoma

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