Effect of first dimension phase selectivity in online comprehensive two dimensional liquid chromatography (LC×LC)

Haiwei Gu; Yuan Huang; Marcelo Filgueira; Peter W. Carr

doi:10.1016/j.chroma.2011.07.063

Effect of first dimension phase selectivity in online comprehensive two dimensional liquid chromatography (LC×LC)

Haiwei Gu, Yuan Huang, Marcelo Filgueira, Peter W. Carr

Chemistry (Twin Cities)

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

In this study, we examined the effect of first dimension column selectivity in reversed phase (RP) online comprehensive two dimensional liquid chromatography (LC×LC). The second dimension was always a carbon clad metal oxide reversed phase material. The hydrophobic subtraction model (HSM) and the related phase selective triangles were used to guide the selection of six different RP first dimension columns. Various kinds of samples were investigated and thus two different elution conditions were needed to cause full elution from the first dimension columns. We compared LC×LC chromatograms, contours plots, and f_coverage plots by measuring peak capacities, peak numbers, relative spatial coverage, correlation values, etc. The major finding of this study is that the carbon phase due to its rather different selectivity from other reversed phases is reasonably orthogonal to a variety of common types of bonded reversed phases. Thus quite surprisingly the six different first dimension stationary phases all showed generally similar separation patterns when paired to the second dimension carbon phase. This result greatly simplifies the task of choosing the correct pair of phases for RP×RP.

Original language	English (US)
Pages (from-to)	6675-6687
Number of pages	13
Journal	Journal of Chromatography A
Volume	1218
Issue number	38
DOIs	https://doi.org/10.1016/j.chroma.2011.07.063
State	Published - Sep 23 2011

Bibliographical note

Funding Information:
This work was supported by grants from the NIH ( GM 054585 ) and the NSF ( CHE-0911516 ). The authors would like to thank Agilent Technologies, Supelco, and ZirChrom Separations for their generous donation of columns. In addition, the authors acknowledge Prof. Jerry Cohen's research group, Prof. Devin G. Peterson's research group, and Dr. Fred Schendel all at the University of Minnesota for providing several samples used in this study.

Keywords

Carbon stationary phases
Hydrophobic subtraction method
LC×LC method development
Reversed phase
Selectivity
Two dimensional liquid chromatography

Publisher link

10.1016/j.chroma.2011.07.063

Find It

Find It at U of M Libraries

Cite this

@article{f8129a6cd25642a5afcae34b82940876,

title = "Effect of first dimension phase selectivity in online comprehensive two dimensional liquid chromatography (LC×LC)",

abstract = "In this study, we examined the effect of first dimension column selectivity in reversed phase (RP) online comprehensive two dimensional liquid chromatography (LC×LC). The second dimension was always a carbon clad metal oxide reversed phase material. The hydrophobic subtraction model (HSM) and the related phase selective triangles were used to guide the selection of six different RP first dimension columns. Various kinds of samples were investigated and thus two different elution conditions were needed to cause full elution from the first dimension columns. We compared LC×LC chromatograms, contours plots, and fcoverage plots by measuring peak capacities, peak numbers, relative spatial coverage, correlation values, etc. The major finding of this study is that the carbon phase due to its rather different selectivity from other reversed phases is reasonably orthogonal to a variety of common types of bonded reversed phases. Thus quite surprisingly the six different first dimension stationary phases all showed generally similar separation patterns when paired to the second dimension carbon phase. This result greatly simplifies the task of choosing the correct pair of phases for RP×RP.",

keywords = "Carbon stationary phases, Hydrophobic subtraction method, LC×LC method development, Reversed phase, Selectivity, Two dimensional liquid chromatography",

author = "Haiwei Gu and Yuan Huang and Marcelo Filgueira and Carr, {Peter W.}",

note = "Funding Information: This work was supported by grants from the NIH ( GM 054585 ) and the NSF ( CHE-0911516 ). The authors would like to thank Agilent Technologies, Supelco, and ZirChrom Separations for their generous donation of columns. In addition, the authors acknowledge Prof. Jerry Cohen's research group, Prof. Devin G. Peterson's research group, and Dr. Fred Schendel all at the University of Minnesota for providing several samples used in this study. ",

year = "2011",

month = sep,

day = "23",

doi = "10.1016/j.chroma.2011.07.063",

language = "English (US)",

volume = "1218",

pages = "6675--6687",

journal = "Journal of Chromatography A",

issn = "0021-9673",

number = "38",

}

TY - JOUR

T1 - Effect of first dimension phase selectivity in online comprehensive two dimensional liquid chromatography (LC×LC)

AU - Gu, Haiwei

AU - Huang, Yuan

AU - Filgueira, Marcelo

AU - Carr, Peter W.

N1 - Funding Information: This work was supported by grants from the NIH ( GM 054585 ) and the NSF ( CHE-0911516 ). The authors would like to thank Agilent Technologies, Supelco, and ZirChrom Separations for their generous donation of columns. In addition, the authors acknowledge Prof. Jerry Cohen's research group, Prof. Devin G. Peterson's research group, and Dr. Fred Schendel all at the University of Minnesota for providing several samples used in this study.

PY - 2011/9/23

Y1 - 2011/9/23

N2 - In this study, we examined the effect of first dimension column selectivity in reversed phase (RP) online comprehensive two dimensional liquid chromatography (LC×LC). The second dimension was always a carbon clad metal oxide reversed phase material. The hydrophobic subtraction model (HSM) and the related phase selective triangles were used to guide the selection of six different RP first dimension columns. Various kinds of samples were investigated and thus two different elution conditions were needed to cause full elution from the first dimension columns. We compared LC×LC chromatograms, contours plots, and fcoverage plots by measuring peak capacities, peak numbers, relative spatial coverage, correlation values, etc. The major finding of this study is that the carbon phase due to its rather different selectivity from other reversed phases is reasonably orthogonal to a variety of common types of bonded reversed phases. Thus quite surprisingly the six different first dimension stationary phases all showed generally similar separation patterns when paired to the second dimension carbon phase. This result greatly simplifies the task of choosing the correct pair of phases for RP×RP.

AB - In this study, we examined the effect of first dimension column selectivity in reversed phase (RP) online comprehensive two dimensional liquid chromatography (LC×LC). The second dimension was always a carbon clad metal oxide reversed phase material. The hydrophobic subtraction model (HSM) and the related phase selective triangles were used to guide the selection of six different RP first dimension columns. Various kinds of samples were investigated and thus two different elution conditions were needed to cause full elution from the first dimension columns. We compared LC×LC chromatograms, contours plots, and fcoverage plots by measuring peak capacities, peak numbers, relative spatial coverage, correlation values, etc. The major finding of this study is that the carbon phase due to its rather different selectivity from other reversed phases is reasonably orthogonal to a variety of common types of bonded reversed phases. Thus quite surprisingly the six different first dimension stationary phases all showed generally similar separation patterns when paired to the second dimension carbon phase. This result greatly simplifies the task of choosing the correct pair of phases for RP×RP.

KW - Carbon stationary phases

KW - Hydrophobic subtraction method

KW - LC×LC method development

KW - Reversed phase

KW - Selectivity

KW - Two dimensional liquid chromatography

UR - http://www.scopus.com/inward/record.url?scp=81155162477&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=81155162477&partnerID=8YFLogxK

U2 - 10.1016/j.chroma.2011.07.063

DO - 10.1016/j.chroma.2011.07.063

M3 - Article

C2 - 21840009

AN - SCOPUS:81155162477

SN - 0021-9673

VL - 1218

SP - 6675

EP - 6687

JO - Journal of Chromatography A

JF - Journal of Chromatography A

IS - 38

ER -

Effect of first dimension phase selectivity in online comprehensive two dimensional liquid chromatography (LC×LC)

Abstract

Bibliographical note

Keywords

Publisher link

Find It

Other files and links

Fingerprint

Cite this