Effect of diurnal photosynthetic activity on the fine structure of amylopectin from normal and waxy barley starch

Avi Goldstein, George Annor, Andreas Blennow, Eric Bertoft

Research output: Contribution to journalArticle

Abstract

The impact of diurnal photosynthetic activity on the fine structure of the amylopectin fraction of starch synthesized by normal barley (NBS) and waxy barley (WBS), the latter completely devoid of amylose biosynthesis, was determined following the cultivation under normal diurnal or constant light growing conditions. The amylopectin fine structures were analysed by characterizing its unit chain length profiles after enzymatic debranching as well as its φ,β-limit dextrins and its clusters and building blocks after their partial and complete hydrolysis with α-amylase from Bacillus amyloliquefaciens, respectively. Regardless of lighting conditions, no structural effects were found when comparing both the amylopectin side-chain distribution and the internal chain fragments of these amylopectins. However, the diurnally grown NBS and WBS both showed larger amylopectin clusters and these had lower branching density and longer average chain lengths than clusters derived from plants grown under constant light conditions. Amylopectin clusters from diurnally grown plants also consisted of a greater number of building blocks, and shorter inter-block chain lengths compared to clusters derived from plants grown under constant light. Our data demonstrate that the diurnal light regime influences the fine structure of the amylopectin component both in amylose and non-amylose starch granules.

Original languageEnglish (US)
Pages (from-to)924-932
Number of pages9
JournalInternational Journal of Biological Macromolecules
Volume102
DOIs
StatePublished - Sep 1 2017

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Amylopectin
Hordeum
Starch
Cycloheximide
Amylose
Glucagon-Like Peptides
Dextrins
alpha-Amylases
Lighting
Bacillus
Hydrolysis
Brain Death
Strabismus
Biosynthesis

Keywords

  • Amylopectin structure
  • Barley starch
  • Cluster structure
  • Diurnal activity

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology

MeSH PubMed subject areas

  • Journal Article

Cite this

Effect of diurnal photosynthetic activity on the fine structure of amylopectin from normal and waxy barley starch. / Goldstein, Avi; Annor, George; Blennow, Andreas; Bertoft, Eric.

In: International Journal of Biological Macromolecules, Vol. 102, 01.09.2017, p. 924-932.

Research output: Contribution to journalArticle

Goldstein, Avi; Annor, George; Blennow, Andreas; Bertoft, Eric / Effect of diurnal photosynthetic activity on the fine structure of amylopectin from normal and waxy barley starch.

In: International Journal of Biological Macromolecules, Vol. 102, 01.09.2017, p. 924-932.

Research output: Contribution to journalArticle

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