Abstract
Objective: To study on effect of concentration of catalpol and 5-hydroxy methyl-2-furaldehyde (5-HMF) from Rehmanniae Radix at various processing. Method: The Rehmanniae Radix was dried and prepared from the steaming process with 10% ethanol, 50% ethanol at 90 °C and 100 °C each other. And the changes of catalpol and 5-HMF was determinated. The extraction of 5-HMF and catalpol was sonicated in 30% methanol for 2 h. The analysis of 5-HMF and catalpol was conducted by HPLC with reversed-phase C-18 column and detected under UV 284 nm, 204 nm. Elution was carried out at 1.0 mL min-1 with 3% acetonitrile. Result: From this analysis, we found out that the content of catalpol was decreased with the number of processing times, and content of 5-HMF was increased with the number of processing times at various processing. The temperature and concentration of ethanol can effect on content of catalpol and 5-HMF at processing. The Cooked Rehmanniae Radix processed at 100 °C, 10% ethanol is best. And the content of 5-HMF processed for more than 7 times was accorded with standard of Korea phamcopoetia. Conclusion: Analyze the effect of concentration of catalpol and 5-HMF from Rehmanniae Radix at various processing, and provide the foundation for further study.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1155-1157 |
| Number of pages | 3 |
| Journal | Zhongguo Zhongyao Zazhi |
| Volume | 32 |
| Issue number | 12 |
| State | Published - Jun 2007 |
| Externally published | Yes |
Keywords
- 5-hydroxymethyl-2-furaldehyde
- Catalpol
- HPLC
- Rehmanniae Radix