Effect of common Brassica vegetables (Brussels sprouts and red cabbage) on the development of preneoplastic lesions induced by 2-amino-3-methylimidazo[4,5- f]quinoline (IQ) in liver and colon of Fischer 344 rats

Maria Uhl, Fekadu Kassie, Sylvie Rabot, Bettina Grasl-Kraupp, Asima Chakraborty, Brenda Laky, Michael Kundi, Siegfried Knasmüller

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28 Citations (Scopus)

Abstract

Aim of the present study was the investigation of effects of juices from commonly consumed Brassica vegetables (two cultivars of Brussels sprouts and two cultivars of red cabbage) on formation and development of preneoplastic lesions in colons (aberrant crypt foci, ACF) and livers (glutathione-S- transferase placental form, GST-P+) in male F344 rats. The foci were induced by 2-amino-3-methylimidazo[4,5-f]quinoline (IQ), a widespread carcinogenic heterocyclic aromatic amine which is found in fried meats. Recently, we reported on pronounced protective effects in the two-organ foci model when the vegetable juices were given during the carcinogen treatment but several findings by other groups indicated that breakdown products of glucosinolates contained in Brassica vegetables cause tumour promotion in various organs of laboratory rodents. In the present study, the animals received the juices in the drinking water (5%) over a period of 20 days after treatment with IQ (100mg/kg bw on 10 alternate days). To increase the foci yield (which facilitates the detection of modifying effects), the animals were fed with a modified (high fat, fibre free) AIN-76 diet. With exception of the sprout variety "Cyrus", all juices lowered the number of GST-P + foci as well as the foci area in the liver, but none of these effects was statistically significant. In the colon, none of the juices had an impact on crypt multiplicity (number of crypts/focus), whereas the number of ACF was decreased; only with the sprout variety Maximus the protective effect was significant (reduction 49%). The present findings show that administration of vegetable juices to the animals after the carcinogen does not increase the number and size of IQ-induced preneoplastic lesions in liver and colon.

Original languageEnglish (US)
Pages (from-to)225-230
Number of pages6
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume802
Issue number1
DOIs
StatePublished - Mar 25 2004

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2-amino-3-methylimidazo(4,5-f)quinoline
Brassica
Inbred F344 Rats
Vegetables
Aberrant Crypt Foci
Liver
Rats
Colon
Carcinogens
Animals
Glucosinolates
Glutathione Transferase
Drinking Water
Meat
Amines
Meats
Rodentia
Nutrition
Fats
Diet

Keywords

  • 2-Amino-3-methylimidazo[4,5-f]quinoline
  • Brassica
  • Heterocyclic aromatic amines

Cite this

Effect of common Brassica vegetables (Brussels sprouts and red cabbage) on the development of preneoplastic lesions induced by 2-amino-3-methylimidazo[4,5- f]quinoline (IQ) in liver and colon of Fischer 344 rats. / Uhl, Maria; Kassie, Fekadu; Rabot, Sylvie; Grasl-Kraupp, Bettina; Chakraborty, Asima; Laky, Brenda; Kundi, Michael; Knasmüller, Siegfried.

In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 802, No. 1, 25.03.2004, p. 225-230.

Research output: Contribution to journalArticle

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AU - Rabot, Sylvie

AU - Grasl-Kraupp, Bettina

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