Effect of collagen cross-linking on quantitative MRI parameters of articular cartilage

J. Rautiainen, M. T. Nieminen, E. N. Salo, H. T. Kokkonen, Silvia Mangia, Shalom Michaeli, O. Gröhn, J. S. Jurvelin, J. Töyräs, M. J. Nissi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Objective To investigate the sensitivity of quantitative magnetic resonance imaging (MRI) parameters to increase of collagen cross-linking in articular cartilage, a factor possibly contributing to the aging-related development of osteoarthritis (OA). The issue has not been widely studied although collagen cross-links may significantly affect the evaluation of cartilage imaging outcome. Design Osteochondral samples (n = 14) were prepared from seven bovine patellae. To induce cross-linking, seven samples were incubated in threose while the other seven served as non-treated controls. The specimens were scanned at 9.4 T for T1, T1Gd (dGEMRIC), T2, adiabatic and continuous wave (CW) T, adiabatic T and T1sat relaxation times. Specimens from adjacent tissue were identically treated and used for reference to determine biomechanical properties, collagen, proteoglycan and cross-link contents, fixed charge density (FCD), collagen fibril anisotropy and water concentration of cartilage. Results In the threose-treated sample group, cross-links (pentosidine, lysyl pyridinoline (LP)), FCD and equilibrium modulus were significantly (P < 0.05) higher as compared to the non-treated group. Threose treatment resulted in significantly greater T1Gd relaxation time constant (+26%, P < 0.05), although proteoglycan content was not altered. Adiabatic and CW-T were also significantly increased (+16%, +28%, P < 0.05) while pre-contrast T1 was significantly decreased (−10%, P < 0.05) in the threose group. T2, T and T1sat did not change significantly. Conclusion Threose treatment induced collagen cross-linking and changes in the properties of articular cartilage, which were detected by T1, T1Gd and T relaxation time constants. Cross-linking should be considered especially when interpreting the outcome of contrast-enhanced MRI in aging populations.

Original languageEnglish (US)
Pages (from-to)1656-1664
Number of pages9
JournalOsteoarthritis and Cartilage
Volume24
Issue number9
DOIs
StatePublished - Sep 1 2016

Fingerprint

Cartilage
Articular Cartilage
Magnetic resonance
Collagen
Magnetic Resonance Imaging
Imaging techniques
Relaxation time
Proteoglycans
Charge density
Aging of materials
Patella
Anisotropy
Osteoarthritis
erythrose
Tissue
Water
Population

Keywords

  • Articular cartilage
  • Collagen
  • Cross-linking
  • Quantitative MRI
  • Relaxation time
  • Threose

Cite this

Effect of collagen cross-linking on quantitative MRI parameters of articular cartilage. / Rautiainen, J.; Nieminen, M. T.; Salo, E. N.; Kokkonen, H. T.; Mangia, Silvia; Michaeli, Shalom; Gröhn, O.; Jurvelin, J. S.; Töyräs, J.; Nissi, M. J.

In: Osteoarthritis and Cartilage, Vol. 24, No. 9, 01.09.2016, p. 1656-1664.

Research output: Contribution to journalArticle

Rautiainen, J, Nieminen, MT, Salo, EN, Kokkonen, HT, Mangia, S, Michaeli, S, Gröhn, O, Jurvelin, JS, Töyräs, J & Nissi, MJ 2016, 'Effect of collagen cross-linking on quantitative MRI parameters of articular cartilage', Osteoarthritis and Cartilage, vol. 24, no. 9, pp. 1656-1664. https://doi.org/10.1016/j.joca.2016.04.017
Rautiainen, J. ; Nieminen, M. T. ; Salo, E. N. ; Kokkonen, H. T. ; Mangia, Silvia ; Michaeli, Shalom ; Gröhn, O. ; Jurvelin, J. S. ; Töyräs, J. ; Nissi, M. J. / Effect of collagen cross-linking on quantitative MRI parameters of articular cartilage. In: Osteoarthritis and Cartilage. 2016 ; Vol. 24, No. 9. pp. 1656-1664.
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abstract = "Objective To investigate the sensitivity of quantitative magnetic resonance imaging (MRI) parameters to increase of collagen cross-linking in articular cartilage, a factor possibly contributing to the aging-related development of osteoarthritis (OA). The issue has not been widely studied although collagen cross-links may significantly affect the evaluation of cartilage imaging outcome. Design Osteochondral samples (n = 14) were prepared from seven bovine patellae. To induce cross-linking, seven samples were incubated in threose while the other seven served as non-treated controls. The specimens were scanned at 9.4 T for T1, T1Gd (dGEMRIC), T2, adiabatic and continuous wave (CW) T1ρ, adiabatic T2ρ and T1sat relaxation times. Specimens from adjacent tissue were identically treated and used for reference to determine biomechanical properties, collagen, proteoglycan and cross-link contents, fixed charge density (FCD), collagen fibril anisotropy and water concentration of cartilage. Results In the threose-treated sample group, cross-links (pentosidine, lysyl pyridinoline (LP)), FCD and equilibrium modulus were significantly (P < 0.05) higher as compared to the non-treated group. Threose treatment resulted in significantly greater T1Gd relaxation time constant (+26{\%}, P < 0.05), although proteoglycan content was not altered. Adiabatic and CW-T1ρ were also significantly increased (+16{\%}, +28{\%}, P < 0.05) while pre-contrast T1 was significantly decreased (−10{\%}, P < 0.05) in the threose group. T2, T2ρ and T1sat did not change significantly. Conclusion Threose treatment induced collagen cross-linking and changes in the properties of articular cartilage, which were detected by T1, T1Gd and T1ρ relaxation time constants. Cross-linking should be considered especially when interpreting the outcome of contrast-enhanced MRI in aging populations.",
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AU - Nieminen, M. T.

AU - Salo, E. N.

AU - Kokkonen, H. T.

AU - Mangia, Silvia

AU - Michaeli, Shalom

AU - Gröhn, O.

AU - Jurvelin, J. S.

AU - Töyräs, J.

AU - Nissi, M. J.

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N2 - Objective To investigate the sensitivity of quantitative magnetic resonance imaging (MRI) parameters to increase of collagen cross-linking in articular cartilage, a factor possibly contributing to the aging-related development of osteoarthritis (OA). The issue has not been widely studied although collagen cross-links may significantly affect the evaluation of cartilage imaging outcome. Design Osteochondral samples (n = 14) were prepared from seven bovine patellae. To induce cross-linking, seven samples were incubated in threose while the other seven served as non-treated controls. The specimens were scanned at 9.4 T for T1, T1Gd (dGEMRIC), T2, adiabatic and continuous wave (CW) T1ρ, adiabatic T2ρ and T1sat relaxation times. Specimens from adjacent tissue were identically treated and used for reference to determine biomechanical properties, collagen, proteoglycan and cross-link contents, fixed charge density (FCD), collagen fibril anisotropy and water concentration of cartilage. Results In the threose-treated sample group, cross-links (pentosidine, lysyl pyridinoline (LP)), FCD and equilibrium modulus were significantly (P < 0.05) higher as compared to the non-treated group. Threose treatment resulted in significantly greater T1Gd relaxation time constant (+26%, P < 0.05), although proteoglycan content was not altered. Adiabatic and CW-T1ρ were also significantly increased (+16%, +28%, P < 0.05) while pre-contrast T1 was significantly decreased (−10%, P < 0.05) in the threose group. T2, T2ρ and T1sat did not change significantly. Conclusion Threose treatment induced collagen cross-linking and changes in the properties of articular cartilage, which were detected by T1, T1Gd and T1ρ relaxation time constants. Cross-linking should be considered especially when interpreting the outcome of contrast-enhanced MRI in aging populations.

AB - Objective To investigate the sensitivity of quantitative magnetic resonance imaging (MRI) parameters to increase of collagen cross-linking in articular cartilage, a factor possibly contributing to the aging-related development of osteoarthritis (OA). The issue has not been widely studied although collagen cross-links may significantly affect the evaluation of cartilage imaging outcome. Design Osteochondral samples (n = 14) were prepared from seven bovine patellae. To induce cross-linking, seven samples were incubated in threose while the other seven served as non-treated controls. The specimens were scanned at 9.4 T for T1, T1Gd (dGEMRIC), T2, adiabatic and continuous wave (CW) T1ρ, adiabatic T2ρ and T1sat relaxation times. Specimens from adjacent tissue were identically treated and used for reference to determine biomechanical properties, collagen, proteoglycan and cross-link contents, fixed charge density (FCD), collagen fibril anisotropy and water concentration of cartilage. Results In the threose-treated sample group, cross-links (pentosidine, lysyl pyridinoline (LP)), FCD and equilibrium modulus were significantly (P < 0.05) higher as compared to the non-treated group. Threose treatment resulted in significantly greater T1Gd relaxation time constant (+26%, P < 0.05), although proteoglycan content was not altered. Adiabatic and CW-T1ρ were also significantly increased (+16%, +28%, P < 0.05) while pre-contrast T1 was significantly decreased (−10%, P < 0.05) in the threose group. T2, T2ρ and T1sat did not change significantly. Conclusion Threose treatment induced collagen cross-linking and changes in the properties of articular cartilage, which were detected by T1, T1Gd and T1ρ relaxation time constants. Cross-linking should be considered especially when interpreting the outcome of contrast-enhanced MRI in aging populations.

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