Effect of chorioamnionitis on regulatory T cells in moderate/late preterm neonates

Cesar M. Rueda, Casey B. Wells, Tate Gisslen, Alan H. Jobe, Suhas G. Kallapur, Claire A. Chougnet

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

Regulatory T-cells (Treg) have a protective role for the control of immune activation and tissue damage. The effects of chorioamnionitis (chorio) on Treg in moderate/late preterm newborns are not known. We hypothesized that infants exposed to chorio would have decreased Treg frequency and/or function.We isolated mononuclear cells from adult peripheral blood and cord blood from term and moderate/late preterm infants who were classified for severity of chorio exposure. Mononuclear cells were analyzed by flow cytometry for Treg frequency and phenotype. Treg suppression of activation of conventional T-cells (Tcon) was also quantified.Treg frequencies were similar in all groups of neonates, but lower than that found in adults. Newborn Treg had a naïve phenotype, with decreased levels of CD45RO, HLA-DR, CD39 and TIGIT compared to adult Treg and chorio did not affect the phenotype. Treg from preterm newborns exposed to severe chorio had higher expression of Ki67 compared to the other groups. Treg from preterm newborns were less suppressive than Treg from adults or term, and the level of suppression was reduced with severe chorio.Relative to term, Treg frequency and phenotype were not affected by prematurity and chorio but their functionality was decreased. Lower Treg activity may contribute to inflammation in newborns that is often associated with chorioamnionitis.

Original languageEnglish (US)
Pages (from-to)65-73
Number of pages9
JournalHuman Immunology
Volume76
Issue number1
DOIs
StatePublished - Jan 1 2015

Keywords

  • Fetal inflammation
  • Full term newborn
  • Prematurity
  • Regulatory T cell
  • Suppression

Fingerprint Dive into the research topics of 'Effect of chorioamnionitis on regulatory T cells in moderate/late preterm neonates'. Together they form a unique fingerprint.

Cite this