Effect of bifidobacteria delivered in fluid milk on human colonic gas production and microfiora

M. Amann, M. Martini, F. Biista, L. Brady

Research output: Contribution to journalArticlepeer-review

Abstract

There are in vivo and in vitro data which suggest that bif idobacteria delivered in fluid milk or yogurt depress populations of undesirable gas producing organisms such as clostridia. The objective of this pilot study was to determine colonie H, gas production in humans consuming fluid skim milk with added bifidobacteria. Three healthy lactose tolerant, non-methane producing, non-smoking males who had not consumed bifidobacteria-containing products or used antibiotics for more than four months prior to the study were the subjects. Subjects consumed fluid skim milk inoculated with a total of 1010 food grade bifidobacteria daily throughout the 10-14d feeding period. Breath H; samples were collected at baseline and at the end of the feeding period. The samples were analyzed for H2 and CO2 and observed H2 values were corrected for atmospheric contamination of alveolar air. During the faifidobacteria consumption period total H2 gas production over the 8hr gas collection period decreased for each subject, though the difference was not significant compared to baseline. Fecal samples were collected at baseline, daily throughout the feeding period, and 11-13d following the feeding trial. Fresh samples were homogenized and plated anaerobically on selective media for bifidobacteria and Clostridiutn perfringens. At the end of the feeding period, bifidobacteria populations were elevated above baseline values in all subjects, as was the ratio of bifidobacteria to C. perfringens. These results suggest that bifidobacteria consumption may reduce gas production through alteration in colonie bacteria populations. MN-SD Dairy Foods Research Center and USDA.

Original languageEnglish (US)
Pages (from-to)A796
JournalFASEB Journal
Volume10
Issue number3
StatePublished - Dec 1 1996

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