Abstract
Semen was collected from eight dogs after SC administration of 0.1 mg/kg PGF2alpha or 0.6 mL 0.9% NaCl solution 15 min prior to collection in the presence or absence of an estrous teaser bitch (switchback design; all dogs given all four treatments in random sequence). There were more spermatozoa (P = 0.02) in ejaculates collected after administration of PGF2alpha in the presence of an estrous teaser bitch ((852 ± 736) × 106, mean ± S.D.) than in ejaculates collected in saline-treated dogs in the absence of a teaser bitch ((371 ± 620) × 106). However, the number of spermatozoa in the ejaculate of dogs given PGF2alpha in the absence of a teaser bitch and in dogs given saline in the presence of a teaser bitch ((556 ± 494 and 600 ± 622) × 106, respectively) were not significantly different from each other or from the other two groups. The percentage of morphologically normal spermatozoa did not vary by treatment (P = 0.51). In conclusion, treatment with PGF2alpha and presence of a teaser bitch had an additive effect on the number of spermatozoa. This, coupled with relatively minor side-effects, suggests this is a useful technique to increase number of spermatozoa in a single canine ejaculate.
Original language | English (US) |
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Pages (from-to) | 255-258 |
Number of pages | 4 |
Journal | Theriogenology |
Volume | 67 |
Issue number | 2 |
DOIs | |
State | Published - Jan 15 2007 |
Bibliographical note
Funding Information:The authors thank the American Kennel Club Canine Health Foundation for supporting this research, Jane Anderson for providing subjects for the study, and Dr. Carl Jessen for statistics assistance.
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
Keywords
- Canine
- Prostaglandin
- Semen collection
- Semen quality
- Spermatozoa