Semen was collected from eight dogs after SC administration of 0.1 mg/kg PGF2alpha or 0.6 mL 0.9% NaCl solution 15 min prior to collection in the presence or absence of an estrous teaser bitch (switchback design; all dogs given all four treatments in random sequence). There were more spermatozoa (P = 0.02) in ejaculates collected after administration of PGF2alpha in the presence of an estrous teaser bitch ((852 ± 736) × 106, mean ± S.D.) than in ejaculates collected in saline-treated dogs in the absence of a teaser bitch ((371 ± 620) × 106). However, the number of spermatozoa in the ejaculate of dogs given PGF2alpha in the absence of a teaser bitch and in dogs given saline in the presence of a teaser bitch ((556 ± 494 and 600 ± 622) × 106, respectively) were not significantly different from each other or from the other two groups. The percentage of morphologically normal spermatozoa did not vary by treatment (P = 0.51). In conclusion, treatment with PGF2alpha and presence of a teaser bitch had an additive effect on the number of spermatozoa. This, coupled with relatively minor side-effects, suggests this is a useful technique to increase number of spermatozoa in a single canine ejaculate.
|Original language||English (US)|
|Number of pages||4|
|State||Published - Jan 15 2007|
Bibliographical noteFunding Information:
The authors thank the American Kennel Club Canine Health Foundation for supporting this research, Jane Anderson for providing subjects for the study, and Dr. Carl Jessen for statistics assistance.
Copyright 2008 Elsevier B.V., All rights reserved.
- Semen collection
- Semen quality