The effect of acute uremia on stores of pancreatic insulin was examined. Four groups of rats were studied: nephrectomized rats on a special low nitrogen diet, sham-nephrectomized rats on the same diet, sham-nephrectomized rats on a normal diet, and normal rats on a normal diet. Uremia created by nephrectomy was allowed to continue for 6 days at which time animals were sacrificed. A period of 10 days of diet equilibration was done prior to nephrectomy. Total body weight, pancreas and liver weights declined in sham-operated and nephrectomized rats mostly attributable to the diet. In uremic rats, however, heart weights increased. Insulin expressed as U/g pancreas, total insulin per animal, U/100 g body weight, or as per cent of protein was significantly depressed in the uremic rats compared to sham-operated special diet controls.
Bibliographical noteFunding Information:
From the Department of Medicine, Hennepin County General Hospital, The North Star Research and Development Institute, and the University of Minnesota Medical School, Minneapolis, Minn. Received for publication July I, 1971. Supported in part by NIH Grant AM 11739 and by the North Star Research and Development Institute. ARNOLD W. LINDALL, M.D., PH.D.: Assistant Professor of Medicine, Hennepin County General Hospital, Minneapolis, Minn. ROBERT L. SORENSON. PH.D.: Assistant Professor of Anatomy, University of Minnesota, Minneapolis, Minn. SIMON L. COHEN, M.D.: Staff Physician, St. Mary’s Hospital, London, England. HAROLD A. NASH, PH.D.: Investigator. North Star Research and Development Institute, Minneapolis, Minn. RAFI YOUNOSZAI: Instructor of Anatomy, University of Minnesota, Minneapolis, Minn.