Effect of γ ray irradiation of different doses on glioma cell lines culture in vitro and the protection of γ ray irradiation to neurological function

Aim: To compare the effects of different γ irradiation doses on two glioma cell lines and establish a biological model of glioma cell lines C6 and SHG-44 apoptosis induced by γ irradiation, to explore the effect of γ ray irradiation on glioma cell cycle. Methods: C6 and SHG-44 cells were both divided into 6 groups: 4, 8, 16, 32, 64 Gy and negative control group, and were cultured for 48 h after γ ray irradiation. A flow cytometry was carried out to determine the apoptosis and analyze the changes of cell cycles. Results: γ ray irradiation of different doses could induce the apoptosis of C6 and SHG-44 glioma cell. The apoptosis ratios of C6 cells in the control group, 4, 8, 16, 32 and 64 Gy groups were (3.1 ± 0.5)%, (16.7 ± 0.6)%, (27.9 ± 0.8)%, (27.3 ± 0.4)%, (33.8 ± 0.7)%, (36.7± 0.7)%, those in the irradiation groups were significantly higher than that in the control group(t = 30.16 - 67.65, P < 0.01). The apoptosis ratios of SHG-44 cells in the control group, 4, 8, 16, 32 and 64 Gy groups 48 h after irradiation were significantly higher than that in the control group(t = 35.51 - 103.79, P < 0.01), but there were no significant differences in the increase of cell necrosis between the two groups(P > 0.05). The SHG-44 cells were more sensitive to ionizing radiation. The necrosis ratios were positively correlated to the doses of the two kinds of cells in the G0/G1 period. The necrosis ratios were negatively correlated to the doses in the S period. Conclusion: An ideal model of glioma cells apoptosis can be well established by γ ray irradiation to C6 and SHG-44 cells. γ ray irradiation can cause G1 period arrest and S period delay in cell cycles of the two cell lines.