Abstract
The c-ski proto-oncogene encodes a transcriptional regulator that has been implicated in the development of different tissues at different times during vertebrate development. We identified two novel paralogues of the c-ski gene family, skiA and skiB in zebrafish (Danio rerio). The skiA protein is maternal and ubiquitous while skiB is zygotic. Overexpression of SkiA or SkiB disrupted gastrulation and resulted in a dorsalized phenotype. In situ analyses suggested that overexpression of Ski leads to a slight expansion of dorsal-axial mesoderm, diminishment or loss of ventral mesoderm and radialization of dorsal neuroectoderm. The dorsalized phenotype could be rescued by the ventral specifying factor, BMP4. These results provide evidence that Ski proteins participate in dorsal-ventral specification of both neuroectoderm and mesoderm. Copyright (C) 2000 Elsevier Science Ireland Ltd.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 147-162 |
| Number of pages | 16 |
| Journal | Mechanisms of Development |
| Volume | 95 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - Jul 1 2000 |
Bibliographical note
Funding Information:We thank Drs S. Hughes for the chicken ski cDNA (KS29) clone, S. Ekker for the zebrafish cDNA library, E. Stavnezer for the G8 anti-chicken Ski monoclonal antibody, D. Grunwald for gsc , J.S. Joly for eve , S. Schulte-Merke for ntl , U. Strahle for axial , T. Jowett for krx -20, L. Zon for GATA-1, B. Riggleman for myoD , A. Fjose for pax 2 and N. Ueno for bmp 4 expression constructs. We give special thanks to Drs E. Stavnezer, J.J. Essner and C.J. Finis, and K.J. Clark for critical comments and discussions on the manuscript. We also thank M. Sanders and D.M. Gartner for their imaging expertise. This work was supported by grants from the USDA (96-35205-3846) and NIH (RO1-RR06625).
Keywords
- Axial mesoderm
- BMP4
- Dorsal-ventral patterning
- Neuroectoderm
- Proto-oncogene
- Zebrafish
- c-ski