TY - JOUR
T1 - Ectonucleotidase and adenosine deaminase as inflammatory marker in dairy cows naturally infected by Dictyocaulus viviparus
AU - da Silva, Aleksandro S.
AU - Baldissera, Matheus D.
AU - Bottari, Nathieli B.
AU - Doleski, Pedro H.
AU - Carmo, Guilherme M.
AU - Schwertz, Claiton I.
AU - Lucca, Neuber J.
AU - Henker, Luan C.
AU - Piva, Manoela M.
AU - Giacomin, Patrícia
AU - Stefani, Lenita M.
AU - Leal, Daniela B.R.
AU - Machado, Gustavo
AU - Mendes, Ricardo E.
PY - 2017/4/1
Y1 - 2017/4/1
N2 - The aim of this study was to evaluate the influence of dictyocaulosis (mild or severe) on enzymes of NTPDase, 5′-nucleotidase, and adenosine deaminase (ADA) of dairy cows naturally infected by Dictyocaulus viviparus. Blood and faeces were collected from 22 dairy cows of the same farm to evaluate NTPDase (ATP and ADP substrate), 5′-nucleotidase, and ADA activities on days 0 (pre-treatment) and 10 (post-treatment). Seric activities of NTPDase (ATP substrate), 5′-nucleotidase, and ADA were lower (P < 0.05) in D. viviparus infected animals compared to uninfected cows. The number of D. viviparus larvae per gram of faeces varied among the animals, and they showed different degrees of severity according to respiratory clinical signs of the disease (cough and nasal discharge). Later, these cows were divided into two groups: those with mild (n = 10) and severe (n = 12) disease. Cows with severe disease showed higher NTPDase activity (ATP substrate) than those with mild disease (P ≤ 0.05). The opposite occurred with NTPDase (ADP substrate), 5′-nucleotidase, and ADA in cows with severe disease, that is, the enzymatic activity of these seric enzymes significantly decreased (P ≤ 0.05) compared to animals with mild disease. Infected animals showed reduced NTPDase activity (ATP and ADP substrate) after treatment. No enzymatic changes were observed for 5′-nucleotidase, and ADA pre- and post-treatment (P > 0.05). Based on these results, we conclude that dictyocaulosis alters NTPDase, 5′-nucleotidase, and ADA activities of cow naturally infected by the parasite, in consequence the enzymes act as inflammatory markers.
AB - The aim of this study was to evaluate the influence of dictyocaulosis (mild or severe) on enzymes of NTPDase, 5′-nucleotidase, and adenosine deaminase (ADA) of dairy cows naturally infected by Dictyocaulus viviparus. Blood and faeces were collected from 22 dairy cows of the same farm to evaluate NTPDase (ATP and ADP substrate), 5′-nucleotidase, and ADA activities on days 0 (pre-treatment) and 10 (post-treatment). Seric activities of NTPDase (ATP substrate), 5′-nucleotidase, and ADA were lower (P < 0.05) in D. viviparus infected animals compared to uninfected cows. The number of D. viviparus larvae per gram of faeces varied among the animals, and they showed different degrees of severity according to respiratory clinical signs of the disease (cough and nasal discharge). Later, these cows were divided into two groups: those with mild (n = 10) and severe (n = 12) disease. Cows with severe disease showed higher NTPDase activity (ATP substrate) than those with mild disease (P ≤ 0.05). The opposite occurred with NTPDase (ADP substrate), 5′-nucleotidase, and ADA in cows with severe disease, that is, the enzymatic activity of these seric enzymes significantly decreased (P ≤ 0.05) compared to animals with mild disease. Infected animals showed reduced NTPDase activity (ATP and ADP substrate) after treatment. No enzymatic changes were observed for 5′-nucleotidase, and ADA pre- and post-treatment (P > 0.05). Based on these results, we conclude that dictyocaulosis alters NTPDase, 5′-nucleotidase, and ADA activities of cow naturally infected by the parasite, in consequence the enzymes act as inflammatory markers.
KW - Dictyocaulosis
KW - Immune response
KW - Purinergic system
KW - Treatment
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U2 - 10.1016/j.cimid.2017.01.001
DO - 10.1016/j.cimid.2017.01.001
M3 - Article
C2 - 28504100
AN - SCOPUS:85013212215
SN - 0147-9571
VL - 51
SP - 9
EP - 13
JO - Comparative Immunology, Microbiology and Infectious Diseases
JF - Comparative Immunology, Microbiology and Infectious Diseases
ER -