ECM-incorporated hydrogels cross-linked via native chemical ligation to engineer stem cell microenvironments

Jangwook P. Jung, Anthony J. Sprangers, John R. Byce, Jing Su, Jayne M. Squirrell, Phillip B. Messersmith, Kevin W. Eliceiri, Brenda M. Ogle

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Limiting the precise study of the biochemical impact of whole molecule extracellular matrix (ECM) proteins on stem cell differentiation is the lack of 3D in vitro models that can accommodate many different types of ECM. Here we sought to generate such a system while maintaining consistent mechanical properties and supporting stem cell survival. To this end, we used native chemical ligation to cross-link poly(ethylene glycol) macromonomers under mild conditions while entrapping ECM proteins (termed ECM composites) and stem cells. Sufficiently low concentrations of ECM were used to maintain constant storage moduli and pore size. Viability of stem cells in composites was maintained over multiple weeks. ECM of composites encompassed stem cells and directed the formation of distinct structures dependent on ECM type. Thus, we introduce a powerful approach to study the biochemical impact of multiple ECM proteins (either alone or in combination) on stem cell behavior.

Original languageEnglish (US)
Pages (from-to)3102-3111
Number of pages10
JournalBiomacromolecules
Volume14
Issue number9
DOIs
StatePublished - Sep 9 2013

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Hydrogels
Stem cells
Engineers
Extracellular Matrix Proteins
Proteins
Composite materials
Polyethylene glycols
Pore size
Elastic moduli
Mechanical properties
Molecules

Cite this

Jung, J. P., Sprangers, A. J., Byce, J. R., Su, J., Squirrell, J. M., Messersmith, P. B., ... Ogle, B. M. (2013). ECM-incorporated hydrogels cross-linked via native chemical ligation to engineer stem cell microenvironments. Biomacromolecules, 14(9), 3102-3111. https://doi.org/10.1021/bm400728e

ECM-incorporated hydrogels cross-linked via native chemical ligation to engineer stem cell microenvironments. / Jung, Jangwook P.; Sprangers, Anthony J.; Byce, John R.; Su, Jing; Squirrell, Jayne M.; Messersmith, Phillip B.; Eliceiri, Kevin W.; Ogle, Brenda M.

In: Biomacromolecules, Vol. 14, No. 9, 09.09.2013, p. 3102-3111.

Research output: Contribution to journalArticle

Jung, JP, Sprangers, AJ, Byce, JR, Su, J, Squirrell, JM, Messersmith, PB, Eliceiri, KW & Ogle, BM 2013, 'ECM-incorporated hydrogels cross-linked via native chemical ligation to engineer stem cell microenvironments', Biomacromolecules, vol. 14, no. 9, pp. 3102-3111. https://doi.org/10.1021/bm400728e
Jung JP, Sprangers AJ, Byce JR, Su J, Squirrell JM, Messersmith PB et al. ECM-incorporated hydrogels cross-linked via native chemical ligation to engineer stem cell microenvironments. Biomacromolecules. 2013 Sep 9;14(9):3102-3111. https://doi.org/10.1021/bm400728e
Jung, Jangwook P. ; Sprangers, Anthony J. ; Byce, John R. ; Su, Jing ; Squirrell, Jayne M. ; Messersmith, Phillip B. ; Eliceiri, Kevin W. ; Ogle, Brenda M. / ECM-incorporated hydrogels cross-linked via native chemical ligation to engineer stem cell microenvironments. In: Biomacromolecules. 2013 ; Vol. 14, No. 9. pp. 3102-3111.
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