Easy-cleaning surfaces functionalized with an interface-binding recombinant lipase

Liting Zhang, Yaofei Sun, Yibing Wang, Xiaoli Wang, Haifeng Zhuang, Chao Chen, Ping Wang

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Surface fabrication is an effective method for functional materials development. This work investigated the use of cellulose-binding domain (CBD) fused detergent lipase for fabrication of easy-cleaning surfaces. As a result, the CBD conjugated Lipase-A (LipA-CBD) demonstrated a multi-layer self-assemble on cotton fabric surface and enhanced hydrophobicity as detected by both scanning electron microscope and water contact angle measurement. Compared to the normal cotton surfaces, such self-assembly bioactive surfaces afforded effective easy-cleaning functionality against both water and lipids based stains with the most significant stain removing ratio as examined through the simulated laundering test. Additionally, this surface assembled LipA-CBD presented good thermal stability with 15 days of half-life detected for 70°C and over 60 days for room temperature. Although there is a gradual decrease in sun irradiation stability and laundering durability, the functionality could be quickly recovered by re-applying the LipA-CBD as the self-assembly coating in the rinsing process. These results presented a green, simple and timesaving method to develop new easy-cleaning cotton fabrics via interfacial self-assembly of biomacromolecules.

Original languageEnglish (US)
Article number110433
JournalColloids and Surfaces B: Biointerfaces
StatePublished - Dec 1 2019

Bibliographical note

Funding Information:
This research was financially supported by National Natural Science Foundation of China ( 21636003 & 51708505 ), and the Science and Technology Department of Zhejiang Province ( 2017C03010 ).

Publisher Copyright:
© 2019


  • Cellulose-binding domain
  • Easy-cleaning surfaces
  • Functional durability
  • Self-assembly
  • Surface fabrication


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