Early assessment of dosimetric and biological differences of total marrow irradiation versus total body irradiation in rodents

Susanta Hui; Yutaka Takahashi; Shernan G. Holtan; Rezvan Azimi; Davis Seelig; Masashi Yagi; Jessie Ingvalson; Parham Alaei; Leslie Sharkey; Behiye Kodal; Nicholas Peterson; Carolyn Meyer; Lindsey Godin; Michael Ehrhardt; Guy Storme; Daohong Zhou; Angela Panoskaltsis-Mortari

doi:10.1016/j.radonc.2017.07.018

Early assessment of dosimetric and biological differences of total marrow irradiation versus total body irradiation in rodents

Susanta Hui, Yutaka Takahashi, Shernan G. Holtan, Rezvan Azimi, Davis Seelig, Masashi Yagi, Jessie Ingvalson, Parham Alaei, Leslie Sharkey, Behiye Kodal, Nicholas Peterson, Carolyn Meyer, Lindsey Godin, Michael Ehrhardt, Guy Storme, Daohong Zhou, Angela Panoskaltsis-Mortari

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Purpose To develop a murine total marrow irradiation (TMI) model in comparison with the total body irradiation (TBI) model. Materials and methods Myeloablative TMI and TBI were administered in mice using a custom jig, and the dosimetric differences between TBI and TMI were evaluated. The early effects of TBI/TMI on bone marrow (BM) and organs were evaluated using histology, FDG-PET, and cytokine production. TMI and TBI with and without cyclophosphamide (Cy) were evaluated for donor cell engraftment and tissue damage early after allogeneic hematopoietic cell transplantation (HCT). Stromal derived factor-1 (SDF-1) expression was evaluated. Results TMI resulted in similar dose exposure to bone and 50% reduction in dose to bystander organs. BM histology was similar between the groups. In the non-HCT model, TMI mice had significantly less acute intestinal and lung injury compared to TBI. In the HCT model, recipients of TMI had significantly less acute intestinal injury and spleen GVHD, but increased early donor cell engraftment and BM:organ SDF-1 ratio compared to TBI recipients. Conclusions The expected BM damage was similar in both models, but the damage to other normal tissues was reduced by TMI. However, BM engraftment was improved in the TMI group compared to TBI, which may be due to enhanced production of SDF-1 in BM relative to other organs after TMI.

Original language	English (US)
Pages (from-to)	468-474
Number of pages	7
Journal	Radiotherapy and Oncology
Volume	124
Issue number	3
DOIs	https://doi.org/10.1016/j.radonc.2017.07.018
State	Published - Sep 2017

Bibliographical note

Funding Information:
This work was supported by the National Institutes of Health ( 1R01CA154491 ). The authors acknowledge and thank Professor (Emeritus) Chang Song ( University of Minnesota ) for helpful advice pertaining to this experiment and Dr. Lucy Brown (City of Hope) on flow cytometry. Appendix A

Funding Information:
This work was supported by the National Institutes of Health (1R01CA154491). The authors acknowledge and thank Professor (Emeritus) Chang Song (University of Minnesota) for helpful advice pertaining to this experiment and Dr. Lucy Brown (City of Hope) on flow cytometry.

Publisher Copyright:
© 2017 The Authors

Keywords

Bone marrow transplant
Graft-versus-host disease
Tissue repair
Total body irradiation
Total marrow irradiation

Publisher link

10.1016/j.radonc.2017.07.018

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5624834

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Hui, S., Takahashi, Y., Holtan, S. G., Azimi, R., Seelig, D., Yagi, M., Ingvalson, J., Alaei, P., Sharkey, L., Kodal, B., Peterson, N., Meyer, C., Godin, L., Ehrhardt, M., Storme, G., Zhou, D., & Panoskaltsis-Mortari, A. (2017). Early assessment of dosimetric and biological differences of total marrow irradiation versus total body irradiation in rodents. Radiotherapy and Oncology, 124(3), 468-474. https://doi.org/10.1016/j.radonc.2017.07.018

Early assessment of dosimetric and biological differences of total marrow irradiation versus total body irradiation in rodents. / Hui, Susanta; Takahashi, Yutaka; Holtan, Shernan G.; Azimi, Rezvan; Seelig, Davis; Yagi, Masashi; Ingvalson, Jessie; Alaei, Parham; Sharkey, Leslie; Kodal, Behiye; Peterson, Nicholas; Meyer, Carolyn; Godin, Lindsey; Ehrhardt, Michael; Storme, Guy; Zhou, Daohong; Panoskaltsis-Mortari, Angela.

In: Radiotherapy and Oncology, Vol. 124, No. 3, 09.2017, p. 468-474.

Research output: Contribution to journal › Article › peer-review

Hui, S, Takahashi, Y, Holtan, SG, Azimi, R, Seelig, D, Yagi, M, Ingvalson, J, Alaei, P, Sharkey, L, Kodal, B, Peterson, N, Meyer, C, Godin, L, Ehrhardt, M, Storme, G, Zhou, D & Panoskaltsis-Mortari, A 2017, 'Early assessment of dosimetric and biological differences of total marrow irradiation versus total body irradiation in rodents', Radiotherapy and Oncology, vol. 124, no. 3, pp. 468-474. https://doi.org/10.1016/j.radonc.2017.07.018

Hui, Susanta ; Takahashi, Yutaka ; Holtan, Shernan G. ; Azimi, Rezvan ; Seelig, Davis ; Yagi, Masashi ; Ingvalson, Jessie ; Alaei, Parham ; Sharkey, Leslie ; Kodal, Behiye ; Peterson, Nicholas ; Meyer, Carolyn ; Godin, Lindsey ; Ehrhardt, Michael ; Storme, Guy ; Zhou, Daohong ; Panoskaltsis-Mortari, Angela. / Early assessment of dosimetric and biological differences of total marrow irradiation versus total body irradiation in rodents. In: Radiotherapy and Oncology. 2017 ; Vol. 124, No. 3. pp. 468-474.

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abstract = "Purpose To develop a murine total marrow irradiation (TMI) model in comparison with the total body irradiation (TBI) model. Materials and methods Myeloablative TMI and TBI were administered in mice using a custom jig, and the dosimetric differences between TBI and TMI were evaluated. The early effects of TBI/TMI on bone marrow (BM) and organs were evaluated using histology, FDG-PET, and cytokine production. TMI and TBI with and without cyclophosphamide (Cy) were evaluated for donor cell engraftment and tissue damage early after allogeneic hematopoietic cell transplantation (HCT). Stromal derived factor-1 (SDF-1) expression was evaluated. Results TMI resulted in similar dose exposure to bone and 50% reduction in dose to bystander organs. BM histology was similar between the groups. In the non-HCT model, TMI mice had significantly less acute intestinal and lung injury compared to TBI. In the HCT model, recipients of TMI had significantly less acute intestinal injury and spleen GVHD, but increased early donor cell engraftment and BM:organ SDF-1 ratio compared to TBI recipients. Conclusions The expected BM damage was similar in both models, but the damage to other normal tissues was reduced by TMI. However, BM engraftment was improved in the TMI group compared to TBI, which may be due to enhanced production of SDF-1 in BM relative to other organs after TMI.",

keywords = "Bone marrow transplant, Graft-versus-host disease, Tissue repair, Total body irradiation, Total marrow irradiation",

author = "Susanta Hui and Yutaka Takahashi and Holtan, {Shernan G.} and Rezvan Azimi and Davis Seelig and Masashi Yagi and Jessie Ingvalson and Parham Alaei and Leslie Sharkey and Behiye Kodal and Nicholas Peterson and Carolyn Meyer and Lindsey Godin and Michael Ehrhardt and Guy Storme and Daohong Zhou and Angela Panoskaltsis-Mortari",

note = "Funding Information: This work was supported by the National Institutes of Health ( 1R01CA154491 ). The authors acknowledge and thank Professor (Emeritus) Chang Song ( University of Minnesota ) for helpful advice pertaining to this experiment and Dr. Lucy Brown (City of Hope) on flow cytometry. Appendix A Funding Information: This work was supported by the National Institutes of Health (1R01CA154491). The authors acknowledge and thank Professor (Emeritus) Chang Song (University of Minnesota) for helpful advice pertaining to this experiment and Dr. Lucy Brown (City of Hope) on flow cytometry. Publisher Copyright: {\textcopyright} 2017 The Authors",

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AU - Hui, Susanta

AU - Takahashi, Yutaka

AU - Holtan, Shernan G.

AU - Azimi, Rezvan

AU - Seelig, Davis

AU - Yagi, Masashi

AU - Ingvalson, Jessie

AU - Alaei, Parham

AU - Sharkey, Leslie

AU - Kodal, Behiye

AU - Peterson, Nicholas

AU - Meyer, Carolyn

AU - Godin, Lindsey

AU - Ehrhardt, Michael

AU - Storme, Guy

AU - Zhou, Daohong

AU - Panoskaltsis-Mortari, Angela

N1 - Funding Information: This work was supported by the National Institutes of Health ( 1R01CA154491 ). The authors acknowledge and thank Professor (Emeritus) Chang Song ( University of Minnesota ) for helpful advice pertaining to this experiment and Dr. Lucy Brown (City of Hope) on flow cytometry. Appendix A Funding Information: This work was supported by the National Institutes of Health (1R01CA154491). The authors acknowledge and thank Professor (Emeritus) Chang Song (University of Minnesota) for helpful advice pertaining to this experiment and Dr. Lucy Brown (City of Hope) on flow cytometry. Publisher Copyright: © 2017 The Authors

PY - 2017/9

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N2 - Purpose To develop a murine total marrow irradiation (TMI) model in comparison with the total body irradiation (TBI) model. Materials and methods Myeloablative TMI and TBI were administered in mice using a custom jig, and the dosimetric differences between TBI and TMI were evaluated. The early effects of TBI/TMI on bone marrow (BM) and organs were evaluated using histology, FDG-PET, and cytokine production. TMI and TBI with and without cyclophosphamide (Cy) were evaluated for donor cell engraftment and tissue damage early after allogeneic hematopoietic cell transplantation (HCT). Stromal derived factor-1 (SDF-1) expression was evaluated. Results TMI resulted in similar dose exposure to bone and 50% reduction in dose to bystander organs. BM histology was similar between the groups. In the non-HCT model, TMI mice had significantly less acute intestinal and lung injury compared to TBI. In the HCT model, recipients of TMI had significantly less acute intestinal injury and spleen GVHD, but increased early donor cell engraftment and BM:organ SDF-1 ratio compared to TBI recipients. Conclusions The expected BM damage was similar in both models, but the damage to other normal tissues was reduced by TMI. However, BM engraftment was improved in the TMI group compared to TBI, which may be due to enhanced production of SDF-1 in BM relative to other organs after TMI.

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KW - Bone marrow transplant

KW - Graft-versus-host disease

KW - Tissue repair

KW - Total body irradiation

KW - Total marrow irradiation

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Early assessment of dosimetric and biological differences of total marrow irradiation versus total body irradiation in rodents

Abstract

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