Abstract
Purpose To develop a murine total marrow irradiation (TMI) model in comparison with the total body irradiation (TBI) model. Materials and methods Myeloablative TMI and TBI were administered in mice using a custom jig, and the dosimetric differences between TBI and TMI were evaluated. The early effects of TBI/TMI on bone marrow (BM) and organs were evaluated using histology, FDG-PET, and cytokine production. TMI and TBI with and without cyclophosphamide (Cy) were evaluated for donor cell engraftment and tissue damage early after allogeneic hematopoietic cell transplantation (HCT). Stromal derived factor-1 (SDF-1) expression was evaluated. Results TMI resulted in similar dose exposure to bone and 50% reduction in dose to bystander organs. BM histology was similar between the groups. In the non-HCT model, TMI mice had significantly less acute intestinal and lung injury compared to TBI. In the HCT model, recipients of TMI had significantly less acute intestinal injury and spleen GVHD, but increased early donor cell engraftment and BM:organ SDF-1 ratio compared to TBI recipients. Conclusions The expected BM damage was similar in both models, but the damage to other normal tissues was reduced by TMI. However, BM engraftment was improved in the TMI group compared to TBI, which may be due to enhanced production of SDF-1 in BM relative to other organs after TMI.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 468-474 |
| Number of pages | 7 |
| Journal | Radiotherapy and Oncology |
| Volume | 124 |
| Issue number | 3 |
| DOIs | |
| State | Published - Sep 1 2017 |
Fingerprint
Keywords
- Bone marrow transplant
- Graft-versus-host disease
- Tissue repair
- Total body irradiation
- Total marrow irradiation
Cite this
Early assessment of dosimetric and biological differences of total marrow irradiation versus total body irradiation in rodents. / Hui, Susanta; Takahashi, Yutaka; Holtan, Shernan G.; Azimi, Rezvan; Seelig, Davis; Yagi, Masashi; Ingvalson, Jessie; Alaei, Parham; Sharkey, Leslie; Kodal, Behiye; Peterson, Nicholas; Meyer, Carolyn; Godin, Lindsey; Ehrhardt, Michael; Storme, Guy; Zhou, Daohong; Panoskaltsis-Mortari, Angela.
In: Radiotherapy and Oncology, Vol. 124, No. 3, 01.09.2017, p. 468-474.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Early assessment of dosimetric and biological differences of total marrow irradiation versus total body irradiation in rodents
AU - Hui, Susanta
AU - Takahashi, Yutaka
AU - Holtan, Shernan G.
AU - Azimi, Rezvan
AU - Seelig, Davis
AU - Yagi, Masashi
AU - Ingvalson, Jessie
AU - Alaei, Parham
AU - Sharkey, Leslie
AU - Kodal, Behiye
AU - Peterson, Nicholas
AU - Meyer, Carolyn
AU - Godin, Lindsey
AU - Ehrhardt, Michael
AU - Storme, Guy
AU - Zhou, Daohong
AU - Panoskaltsis-Mortari, Angela
PY - 2017/9/1
Y1 - 2017/9/1
N2 - Purpose To develop a murine total marrow irradiation (TMI) model in comparison with the total body irradiation (TBI) model. Materials and methods Myeloablative TMI and TBI were administered in mice using a custom jig, and the dosimetric differences between TBI and TMI were evaluated. The early effects of TBI/TMI on bone marrow (BM) and organs were evaluated using histology, FDG-PET, and cytokine production. TMI and TBI with and without cyclophosphamide (Cy) were evaluated for donor cell engraftment and tissue damage early after allogeneic hematopoietic cell transplantation (HCT). Stromal derived factor-1 (SDF-1) expression was evaluated. Results TMI resulted in similar dose exposure to bone and 50% reduction in dose to bystander organs. BM histology was similar between the groups. In the non-HCT model, TMI mice had significantly less acute intestinal and lung injury compared to TBI. In the HCT model, recipients of TMI had significantly less acute intestinal injury and spleen GVHD, but increased early donor cell engraftment and BM:organ SDF-1 ratio compared to TBI recipients. Conclusions The expected BM damage was similar in both models, but the damage to other normal tissues was reduced by TMI. However, BM engraftment was improved in the TMI group compared to TBI, which may be due to enhanced production of SDF-1 in BM relative to other organs after TMI.
AB - Purpose To develop a murine total marrow irradiation (TMI) model in comparison with the total body irradiation (TBI) model. Materials and methods Myeloablative TMI and TBI were administered in mice using a custom jig, and the dosimetric differences between TBI and TMI were evaluated. The early effects of TBI/TMI on bone marrow (BM) and organs were evaluated using histology, FDG-PET, and cytokine production. TMI and TBI with and without cyclophosphamide (Cy) were evaluated for donor cell engraftment and tissue damage early after allogeneic hematopoietic cell transplantation (HCT). Stromal derived factor-1 (SDF-1) expression was evaluated. Results TMI resulted in similar dose exposure to bone and 50% reduction in dose to bystander organs. BM histology was similar between the groups. In the non-HCT model, TMI mice had significantly less acute intestinal and lung injury compared to TBI. In the HCT model, recipients of TMI had significantly less acute intestinal injury and spleen GVHD, but increased early donor cell engraftment and BM:organ SDF-1 ratio compared to TBI recipients. Conclusions The expected BM damage was similar in both models, but the damage to other normal tissues was reduced by TMI. However, BM engraftment was improved in the TMI group compared to TBI, which may be due to enhanced production of SDF-1 in BM relative to other organs after TMI.
KW - Bone marrow transplant
KW - Graft-versus-host disease
KW - Tissue repair
KW - Total body irradiation
KW - Total marrow irradiation
UR - http://www.scopus.com/inward/record.url?scp=85026674277&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85026674277&partnerID=8YFLogxK
U2 - 10.1016/j.radonc.2017.07.018
DO - 10.1016/j.radonc.2017.07.018
M3 - Article
C2 - 28778346
AN - SCOPUS:85026674277
VL - 124
SP - 468
EP - 474
JO - Radiotherapy and Oncology
JF - Radiotherapy and Oncology
SN - 0167-8140
IS - 3
ER -