Dystrophin-related protein is localized to neuromuscular junctions of adult skeletal muscle

Kay Ohlendieck, James M Ervasti, Kiichiro Matsumura, Steven D. Kahl, Cynthia J. Leveille, Kevin P. Campbell

Research output: Contribution to journalArticle

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Abstract

Dystrophin-related protein (DRP) is an autosomal gene product with high homology to dystrophin. We have used highly specific antibodies to the unique C-terminal peptide sequences of DRP and dystrophin to examine the subcellular localization and biochemical properties of DRP in adult skeletal muscle. DRP is enriched in isolated sarcolemma from control and mdx mouse muscle, but is much less abundant than dystrophin. Immunofluorescence microscopy localized DRP almost exclusively to the neuromuscular junction region in rabbit and mouse skeletal muscle, as well as mdx mouse muscle and denervated mouse muscle. DRP is also present in normal size and abundance and localizes to the neuromuscular junction region in muscle from the dystrophic mouse model dy dy. Thus, DRP is a junction-specific membrane cytoskeletal protein that may play an important role in the organization of the postsynaptic membrane of the neuromuscular junction.

Original languageEnglish (US)
Pages (from-to)499-508
Number of pages10
JournalNeuron
Volume7
Issue number3
DOIs
StatePublished - Jan 1 1991

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Utrophin
Neuromuscular Junction
Skeletal Muscle
Dystrophin
Inbred mdx Mouse
Muscles
Sarcolemma
Cytoskeletal Proteins
Fluorescence Microscopy
Membrane Proteins
Rabbits
Peptides
Membranes
Antibodies

Cite this

Ohlendieck, K., Ervasti, J. M., Matsumura, K., Kahl, S. D., Leveille, C. J., & Campbell, K. P. (1991). Dystrophin-related protein is localized to neuromuscular junctions of adult skeletal muscle. Neuron, 7(3), 499-508. https://doi.org/10.1016/0896-6273(91)90301-F

Dystrophin-related protein is localized to neuromuscular junctions of adult skeletal muscle. / Ohlendieck, Kay; Ervasti, James M; Matsumura, Kiichiro; Kahl, Steven D.; Leveille, Cynthia J.; Campbell, Kevin P.

In: Neuron, Vol. 7, No. 3, 01.01.1991, p. 499-508.

Research output: Contribution to journalArticle

Ohlendieck, K, Ervasti, JM, Matsumura, K, Kahl, SD, Leveille, CJ & Campbell, KP 1991, 'Dystrophin-related protein is localized to neuromuscular junctions of adult skeletal muscle', Neuron, vol. 7, no. 3, pp. 499-508. https://doi.org/10.1016/0896-6273(91)90301-F
Ohlendieck, Kay ; Ervasti, James M ; Matsumura, Kiichiro ; Kahl, Steven D. ; Leveille, Cynthia J. ; Campbell, Kevin P. / Dystrophin-related protein is localized to neuromuscular junctions of adult skeletal muscle. In: Neuron. 1991 ; Vol. 7, No. 3. pp. 499-508.
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