Dynamics of the IFT machinery at the ciliary tip

Alexander Chien, Sheng Min Shih, Raqual Bower, Douglas Tritschler, Mary E. Porter, Ahmet Yildiz

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Intraflagellar transport (IFT) is essential for the elongation and maintenance of eukaryotic cilia and flagella. Due to the traffic jam of multiple trains at the ciliary tip, how IFT trains are remodeled in these turnaround zones cannot be determined by conventional imaging. Using PhotoGate, we visualized the full range of movement of single IFT trains and motors in Chlamydomonas flagella. Anterograde trains split apart and IFT complexes mix wiTheach other at the tip to assemble retrograde trains. Dynein-1b is carried to the tip by kinesin-II as inactive cargo on anterograde trains. Unlike dynein-1b, kinesin-II detaches from IFT trains at the tip and diffuses in flagella. As the flagellum grows longer, diffusion delays return of kinesin-II to the basal body, depleting kinesin-II available for anterograde transport. Our results suggest that dissociation of kinesin-II from IFT trains serves as a negative feedback mechanism that facilitates flagellar length control in Chlamydomonas.

Original languageEnglish (US)
Article numbere28606
JournaleLife
Volume6
DOIs
StatePublished - Sep 20 2017

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Flagella
Machinery
Chlamydomonas
Dyneins
Basal Bodies
Cilia
Elongation
Maintenance
kinesin-II
Feedback
Imaging techniques

Cite this

Chien, A., Shih, S. M., Bower, R., Tritschler, D., Porter, M. E., & Yildiz, A. (2017). Dynamics of the IFT machinery at the ciliary tip. eLife, 6, [e28606]. https://doi.org/10.7554/eLife.28606

Dynamics of the IFT machinery at the ciliary tip. / Chien, Alexander; Shih, Sheng Min; Bower, Raqual; Tritschler, Douglas; Porter, Mary E.; Yildiz, Ahmet.

In: eLife, Vol. 6, e28606, 20.09.2017.

Research output: Contribution to journalArticle

Chien, A, Shih, SM, Bower, R, Tritschler, D, Porter, ME & Yildiz, A 2017, 'Dynamics of the IFT machinery at the ciliary tip', eLife, vol. 6, e28606. https://doi.org/10.7554/eLife.28606
Chien A, Shih SM, Bower R, Tritschler D, Porter ME, Yildiz A. Dynamics of the IFT machinery at the ciliary tip. eLife. 2017 Sep 20;6. e28606. https://doi.org/10.7554/eLife.28606
Chien, Alexander ; Shih, Sheng Min ; Bower, Raqual ; Tritschler, Douglas ; Porter, Mary E. ; Yildiz, Ahmet. / Dynamics of the IFT machinery at the ciliary tip. In: eLife. 2017 ; Vol. 6.
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