Dynamics and Topographical Distribution of Surface Glycoproteins during Myoblast Fusion: A Resonance Energy Transfer Study

Brian A. Herman, Salvador M. Fernandez

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

We have investigated changes in topography and lateral translational mobility of concanavalin A (Con A) receptors on the surface of cultured chick muscle cells during the period of myoblast fusion. A temporal correlation between these phenomena and the alteration in membrane fluidity known to occur during this time period is established. Receptor topography and mobility are studied by means of a resonance energy transfer technique employing pyrene- and FITC-Con A conjugates. All measurements are performed through a microscope on single cells. Our results reveal that during the period of myoblast fusion Con A receptors undergo a dramatic redistribution on the cell surface. Furthermore, our data suggest that the changes in membrane fluidity observed during muscle differentiation serve to modulate the lateral mobility of these receptors.

Original languageEnglish (US)
Pages (from-to)3275-3283
Number of pages9
JournalBiochemistry
Volume21
Issue number14
DOIs
StatePublished - Jul 1982

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