Dynamic localization of the nuclear import receptor and its interactions with transport factors

Deanna M. Koepp, Darren H. Wong, Anita H. Corbett, Pamela A. Silver

Research output: Contribution to journalArticle

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Abstract

Characterization of the interactions between soluble factors required for nuclear transport is key to understanding the process of nuclear trafficking. Using a synthetic lethal screen with the rnal-1 strain, we have identified a genetic interaction between Rna1p, a GTPase activating protein required for nuclear transport, and yeast importin-β, a component of the nuclear localization signal receptor. By the use of fusion proteins, we demonstrate that Rna1p physically interacts with importin-β. Mutants in importin-β exhibit in vivo nuclear protein import defects, and importin-β localizes to the nuclear envelope along with other proteins associated with the nuclear pore complex. In addition, we present evidence that importin-α, but not importin-β, mislocalizes to the nucleus in cells where the GTPase Ran is likely to be in the GDP-bound state. We suggest a model of nuclear transport in which Ran-mediated hydrolysis of GTP is necessary for the import of importin-α and the nuclear localization signal-bearing substrate into the nucleus, while exchange of GDP for GTP on Ran is required for the export of both mRNA and importin-α from the nucleus.

Original languageEnglish (US)
Pages (from-to)1163-1176
Number of pages14
JournalJournal of Cell Biology
Volume133
Issue number6
DOIs
StatePublished - Jul 5 1996

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