Dux facilitates post-implantation development, but is not essential for zygotic genome activation

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


Double homeobox genes are unique to eutherian mammals. It has been proposed that the DUXC clade of the double homeobox gene family, which is present in multicopy long tandem arrays, plays an essential role in zygotic genome activation (ZGA). We generated a deletion of the tandem array encoding the DUXC gene of mouse, Double homeobox (Dux), and found it surprisingly to be homozygous viable and fertile. We characterize the embryonic development and ZGA profile of knockout (KO) embryos, finding that zygotic genome activation still occurs, with only modest alterations in 2-cell embryo gene expression, no defect in in vivo preimplantation development, but an increased likelihood of post-implantation developmental failure, leading to correspondingly smaller litter sizes in the KO strain. While all known 2-cell specific Dux target genes are still expressed in the KO, a subset is expressed at lower levels. These include numerous genes involved in methylation, blastocyst development, and trophectoderm/placental development. We propose that rather than driving ZGA, which is a process common throughout the animal kingdom, DUXC genes facilitate a process unique to eutherian mammals, namely the post-implantation development enabled by an invasive placenta.

Original languageEnglish (US)
Pages (from-to)83-93
Number of pages11
JournalBiology of reproduction
Issue number1
StatePublished - Jan 1 2021

Bibliographical note

Publisher Copyright:
© 2020 The Author(s) 2020. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved.


  • DUXC
  • Dux
  • double homeobox
  • post-implantation development
  • zygotic genome activation


Dive into the research topics of 'Dux facilitates post-implantation development, but is not essential for zygotic genome activation'. Together they form a unique fingerprint.

Cite this