Dual-label high-performance liquid chromatographic assay for femtomole levels of benzo[a]pyrene metabolites

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

A dual-label HPLC assay to measure femtomole quantities of ethyl acetate-extractable [3H]benzo[a]pyrene metabolites was developed. 14C-Labeled metabolites of benzo[a]pyrene formed by rat liver 9000g supernatant were used as both internal standards and chromatographic markers. The percentage deviation between assays was determined to be between 11 and 13% for 9,10-dihydro-9,10-dihydroxybenzo[a]pyrene, 7,8-dihydro-7,8-dihydroxybenzo[a]pyrene, benzo[a]pyrene-3,6-quinone, benzo[a]pyrene-1,6-quinone, and 9-hydroxybenzo[a]pyrene, 22% for 4,5-dihydro-4,5-dihydroxybenzo[a]pyrene, and less than 5% for 3-hydroxybenzo[a]pyrene. The detection limit of this assay was between 3 and 10 fmol per metabolite. The application of this technique to the metabolism of [3H]benzo[a]pyrene by microsomes of hamster and human oral cavity tissue is described.

Original languageEnglish (US)
Pages (from-to)442-447
Number of pages6
JournalAnalytical Biochemistry
Volume146
Issue number2
DOIs
StatePublished - May 1 1985

Keywords

  • HPLC, carcinogen metabolites
  • benzo[a]pyrene
  • carcinogen metabolism
  • chemical carcinogenesis
  • dual-label analysis

Fingerprint Dive into the research topics of 'Dual-label high-performance liquid chromatographic assay for femtomole levels of benzo[a]pyrene metabolites'. Together they form a unique fingerprint.

Cite this