TY - JOUR
T1 - Dual-functional peptide conjugated gold nanorods for the detection and photothermal ablation of pathogenic bacteria
AU - Chen, Qingyu
AU - Zhang, Liwei
AU - Feng, Yonghai
AU - Shi, Fan
AU - Wang, Yibing
AU - Wang, Ping
AU - Liu, Lei
PY - 2018
Y1 - 2018
N2 - It is urgent to find an effective and convenient method to detect and kill microbial strains due to the threat of pathogenic bacterial infection and the low effectiveness of antibiotics. In the present work, a dual-functional peptide coupling gold nanoconjugate (Au@P937 NRs) was facilely fabricated by conjugating an adhesion peptide (WGLHTSATNLYLHGGGC, P937) with Au nanorods (Au NRs), of which the peptide P937 was designed by the phage display biopanning technology and showed specific binding affinity to bacteria. After the modification of P937, the longitudinal surface plasmon resonance (LSPR) absorbance peak of the Au@P937 NRs was found to be very sensitive to the variation of bacterial concentration. The Au@P937 NRs exhibited enhanced NIR-based photothermal conversion efficiency. Moreover, the Au@P937 NRs exhibited good biocompatibility. Hence, the Au@P937 NRs could be used as a dual-functional nanoconjugate for the detection and photothermal ablation of bacteria. For the bacterial detection by the Au@P937 NRs, the detection limit could reach 46 cfu mL-1 for E. coli and 89 cfu mL-1 for S. aureus, respectively. Both E. coli and S. aureus could be killed completely in 10 min by the Au@P937 NRs under 808 nm laser irradiation. This work provided a facile, convenient and effective strategy for detecting and sterilizing bacteria.
AB - It is urgent to find an effective and convenient method to detect and kill microbial strains due to the threat of pathogenic bacterial infection and the low effectiveness of antibiotics. In the present work, a dual-functional peptide coupling gold nanoconjugate (Au@P937 NRs) was facilely fabricated by conjugating an adhesion peptide (WGLHTSATNLYLHGGGC, P937) with Au nanorods (Au NRs), of which the peptide P937 was designed by the phage display biopanning technology and showed specific binding affinity to bacteria. After the modification of P937, the longitudinal surface plasmon resonance (LSPR) absorbance peak of the Au@P937 NRs was found to be very sensitive to the variation of bacterial concentration. The Au@P937 NRs exhibited enhanced NIR-based photothermal conversion efficiency. Moreover, the Au@P937 NRs exhibited good biocompatibility. Hence, the Au@P937 NRs could be used as a dual-functional nanoconjugate for the detection and photothermal ablation of bacteria. For the bacterial detection by the Au@P937 NRs, the detection limit could reach 46 cfu mL-1 for E. coli and 89 cfu mL-1 for S. aureus, respectively. Both E. coli and S. aureus could be killed completely in 10 min by the Au@P937 NRs under 808 nm laser irradiation. This work provided a facile, convenient and effective strategy for detecting and sterilizing bacteria.
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U2 - 10.1039/c8tb01835a
DO - 10.1039/c8tb01835a
M3 - Article
C2 - 32254886
AN - SCOPUS:85057572393
VL - 6
SP - 7643
EP - 7651
JO - Journal of Materials Chemistry B
JF - Journal of Materials Chemistry B
SN - 2050-7518
IS - 46
ER -