Dual Carfilzomib and Doxorubicin-Loaded Liposomal Nanoparticles for Synergistic Efficacy in Multiple Myeloma

Jonathan D. Ashley, Charissa J. Quinlan, Valerie A. Schroeder, Mark A. Suckow, Vincenzo J. Pizzuti, Tanyel Kiziltepe, Basar Bilgicer

Research output: Contribution to journalArticlepeer-review

57 Scopus citations

Abstract

Here, we report the synthesis and evaluation of dual drug-loaded nanoparticles as an effective means to deliver carfilzomib and doxorubicin to multiple myeloma tumor cells at their optimal synergistic ratio. First, various molar ratios of carfilzomib to doxorubicin were screened against multiple myeloma cell lines to determine the molar ratio that elicited the greatest synergy using the Chou-Talalay method. The therapeutic agents were then incorporated into liposomes at the optimal synergistic ratio of 1:1 to yield dual drug-loaded nanoparticles with a narrow size range of 115 nm and high reproducibility. Our results demonstrated that the dual drug-loaded liposomes exhibited synergy in vitro and were more efficacious in inhibiting tumor growth in vivo than a combination of free drugs, while at the same time reducing systemic toxicity. Taken together, this study presents the synthesis and preclinical evaluation of dual drug-loaded liposomes containing carfilzomib and doxorubicin for enhanced therapeutic efficacy to improve patient outcome in multiple myeloma.

Original languageEnglish (US)
Pages (from-to)1452-1459
Number of pages8
JournalMolecular Cancer Therapeutics
Volume15
Issue number7
DOIs
StatePublished - Jul 2016

Bibliographical note

Publisher Copyright:
©2016 American Association for Cancer Research.

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