Dual activities of receptor-like kinase OsWAKL21.2 induce immune responses

Kamal Kumar Malukani, Ashish Ranjan, Shiva Jyothi Hota, Hitendra Kumar Patel, Ramesh V. Sonti

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Plant pathogens secrete cell wall-degrading enzymes that degrade various components of the plant cell wall. Plants sense this cell wall damage as a mark of infection and induce immune responses. However, the plant functions that are involved in the elaboration of cell wall damage-induced immune responses remain poorly understood. Transcriptome analysis revealed that a rice (Oryza sativa) receptor-like kinase, WALL-ASSOCIATED KINASE-LIKE21 (OsWAKL21.2), is up-regulated following treatment with either Xanthomonas oryzae pv oryzae (a bacterial pathogen) or lipaseA/esterase (LipA; a cell wall-degrading enzyme of X. oryzae pv oryzae). Overexpression of OsWAKL21.2 in rice induces immune responses similar to those activated by LipA treatment. Down-regulation of OsWAKL21.2 attenuates LipA-mediated immune responses. Heterologous expression of OsWAKL21.2 in Arabidopsis (Arabidopsis thaliana) also activates plant immune responses. OsWAKL21.2 is a dual-activity kinase that has in vitro kinase and guanylate cyclase activities. Interestingly, kinase activity of OsWAKL21.2 is necessary to activate rice immune responses, whereas in Arabidopsis, OsWAKL21.2 guanylate cyclase activity activates these responses. Our study reveals a rice receptor kinase that activates immune responses in two different species via two different mechanisms.

Original languageEnglish (US)
Pages (from-to)1345-1363
Number of pages19
JournalPlant physiology
Volume183
Issue number3
DOIs
StatePublished - Jul 2020
Externally publishedYes

Bibliographical note

Funding Information:
1This work was supported by the 12th 5-year plan project, Plant-Microbe and Soil Interactions, of the Council of Scientific and Industrial Research (grant no. BSC0117) and a J.C. Bose fellowship to R.V.S. by the Department of Science and Technology, Ministry of Science and Technology (grant no. GAP0444). 2Author for contact: sonti@ccmb.res.in. 3Senior author.

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't

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