Draft genome of omphalotus olearius provides a predictive framework for sesquiterpenoid natural product biosynthesis in basidiomycota

Grayson T. Wawrzyn, Maureen B. Quin, Swati Choudhary, Fernando López-Gallego, Claudia Schmidt-Dannert

Research output: Contribution to journalArticlepeer-review

74 Scopus citations

Abstract

The secondary metabolome of Basidiomycota represents a largely uncharacterized source of pharmaceutically relevant natural products. Terpenoids are the primary class of bioactive compounds isolated from mushrooms. The Jack O'Lantern mushroom Omphalotus olearius was identified 50 years ago as a prolific producer of anticancer illudin sesquiterpenoids; however, to date there have been exceptionally few studies into the biosynthesis of these important compounds. Here, we report the draft genome sequence of O. olearius, which reveals a diverse network of sesquiterpene synthases and two metabolic gene clusters associated with illudin biosynthesis. Characterization of the sesquiterpene synthases enabled a comprehensive survey of all currently available Basidiomycota genomes, thereby creating a predictive resource for terpenoid natural product biosynthesis in these organisms. Our results will facilitate discovery and biosynthetic production of unique pharmaceutically relevant bioactive compounds from Basidiomycota.

Original languageEnglish (US)
Pages (from-to)772-783
Number of pages12
JournalChemistry and Biology
Volume19
Issue number6
DOIs
StatePublished - Jun 22 2012

Bibliographical note

Funding Information:
This research was supported by the National Institutes of Health Grant GM080299 (to C.S-D.). G.T.W. was supported by a predoctoral National Institutes of Health traineeship (GM08700).

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