Downregulation of the autophagy protein ATG-7 correlates with distinct sphingolipid profile in MCF-7 cells sensitized to photodamage

Duska Separovic; Ameeta Kelekar; Adi L. Tarca; Jacek Bielawski; David Kessel

doi:10.1117/12.827261

Downregulation of the autophagy protein ATG-7 correlates with distinct sphingolipid profile in MCF-7 cells sensitized to photodamage

Duska Separovic
, Ameeta Kelekar
, Adi L. Tarca
, Jacek Bielawski
, David Kessel

Laboratory Medicine and Pathology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

The objective of this study was to determine the sphingolipid (SL) profile in autophagy-defective cells and overall cell death after PDT with Pc 4 (PDT). Human breast cancer MCF-7 cells with downregulated autophagy protein ATG-7 and their scrambled controls (Scr) were used. Exposure of ATG-7 knockdown cells to PDT led to defective processing of the autophagy marker LC3, and increased overall cell killing. In both cell types PDT evoked an early (2 h) increase in ceramides and dihydroceramides (DHceramides). When the two cell types were compared regarding time (2 and 24 h) and treatment conditions (with and without PDT), the levels of several ceramides and DHceramides were reduced, whereas the concentrations of C14-ceramide, C16-ceramide and C12-DHceramide were higher in ATG-7 knockdown cells. The data imply that the SL profile might be a marker of autophagy-deficiency in cells sensitized to PDT.

Original language	English (US)
Title of host publication	12th World Congress of the International Photodynamic Association - Photodynamic Therapy
Subtitle of host publication	Back to the Future
DOIs	https://doi.org/10.1117/12.827261
State	Published - 2009
Event	12th World Congress of the International Photodynamic Association - Photodynamic Therapy: Back to the Future - Seattle, WA, United States Duration: Jun 11 2009 → Jun 15 2009

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	7380
ISSN (Print)	1605-7422

Other

Other	12th World Congress of the International Photodynamic Association - Photodynamic Therapy: Back to the Future
Country/Territory	United States
City	Seattle, WA
Period	6/11/09 → 6/15/09

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Autophagy
PDT
ceramide
dihydroceramide
sphingosine

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10.1117/12.827261

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Separovic, D., Kelekar, A., Tarca, A. L., Bielawski, J., & Kessel, D. (2009). Downregulation of the autophagy protein ATG-7 correlates with distinct sphingolipid profile in MCF-7 cells sensitized to photodamage. In 12th World Congress of the International Photodynamic Association - Photodynamic Therapy: Back to the Future Article 73805M (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7380). https://doi.org/10.1117/12.827261

Downregulation of the autophagy protein ATG-7 correlates with distinct sphingolipid profile in MCF-7 cells sensitized to photodamage. / Separovic, Duska; Kelekar, Ameeta; Tarca, Adi L. et al.
12th World Congress of the International Photodynamic Association - Photodynamic Therapy: Back to the Future. 2009. 73805M (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7380).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Separovic, D, Kelekar, A, Tarca, AL, Bielawski, J & Kessel, D 2009, Downregulation of the autophagy protein ATG-7 correlates with distinct sphingolipid profile in MCF-7 cells sensitized to photodamage. in 12th World Congress of the International Photodynamic Association - Photodynamic Therapy: Back to the Future., 73805M, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 7380, 12th World Congress of the International Photodynamic Association - Photodynamic Therapy: Back to the Future, Seattle, WA, United States, 6/11/09. https://doi.org/10.1117/12.827261

Separovic D, Kelekar A, Tarca AL, Bielawski J, Kessel D. Downregulation of the autophagy protein ATG-7 correlates with distinct sphingolipid profile in MCF-7 cells sensitized to photodamage. In 12th World Congress of the International Photodynamic Association - Photodynamic Therapy: Back to the Future. 2009. 73805M. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). doi: 10.1117/12.827261

Separovic, Duska ; Kelekar, Ameeta ; Tarca, Adi L. et al. / Downregulation of the autophagy protein ATG-7 correlates with distinct sphingolipid profile in MCF-7 cells sensitized to photodamage. 12th World Congress of the International Photodynamic Association - Photodynamic Therapy: Back to the Future. 2009. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).

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title = "Downregulation of the autophagy protein ATG-7 correlates with distinct sphingolipid profile in MCF-7 cells sensitized to photodamage",

abstract = "The objective of this study was to determine the sphingolipid (SL) profile in autophagy-defective cells and overall cell death after PDT with Pc 4 (PDT). Human breast cancer MCF-7 cells with downregulated autophagy protein ATG-7 and their scrambled controls (Scr) were used. Exposure of ATG-7 knockdown cells to PDT led to defective processing of the autophagy marker LC3, and increased overall cell killing. In both cell types PDT evoked an early (2 h) increase in ceramides and dihydroceramides (DHceramides). When the two cell types were compared regarding time (2 and 24 h) and treatment conditions (with and without PDT), the levels of several ceramides and DHceramides were reduced, whereas the concentrations of C14-ceramide, C16-ceramide and C12-DHceramide were higher in ATG-7 knockdown cells. The data imply that the SL profile might be a marker of autophagy-deficiency in cells sensitized to PDT.",

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author = "Duska Separovic and Ameeta Kelekar and Tarca, \{Adi L.\} and Jacek Bielawski and David Kessel",

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AU - Kelekar, Ameeta

AU - Tarca, Adi L.

AU - Bielawski, Jacek

AU - Kessel, David

PY - 2009

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N2 - The objective of this study was to determine the sphingolipid (SL) profile in autophagy-defective cells and overall cell death after PDT with Pc 4 (PDT). Human breast cancer MCF-7 cells with downregulated autophagy protein ATG-7 and their scrambled controls (Scr) were used. Exposure of ATG-7 knockdown cells to PDT led to defective processing of the autophagy marker LC3, and increased overall cell killing. In both cell types PDT evoked an early (2 h) increase in ceramides and dihydroceramides (DHceramides). When the two cell types were compared regarding time (2 and 24 h) and treatment conditions (with and without PDT), the levels of several ceramides and DHceramides were reduced, whereas the concentrations of C14-ceramide, C16-ceramide and C12-DHceramide were higher in ATG-7 knockdown cells. The data imply that the SL profile might be a marker of autophagy-deficiency in cells sensitized to PDT.

AB - The objective of this study was to determine the sphingolipid (SL) profile in autophagy-defective cells and overall cell death after PDT with Pc 4 (PDT). Human breast cancer MCF-7 cells with downregulated autophagy protein ATG-7 and their scrambled controls (Scr) were used. Exposure of ATG-7 knockdown cells to PDT led to defective processing of the autophagy marker LC3, and increased overall cell killing. In both cell types PDT evoked an early (2 h) increase in ceramides and dihydroceramides (DHceramides). When the two cell types were compared regarding time (2 and 24 h) and treatment conditions (with and without PDT), the levels of several ceramides and DHceramides were reduced, whereas the concentrations of C14-ceramide, C16-ceramide and C12-DHceramide were higher in ATG-7 knockdown cells. The data imply that the SL profile might be a marker of autophagy-deficiency in cells sensitized to PDT.

KW - Autophagy

KW - PDT

KW - ceramide

KW - dihydroceramide

KW - sphingosine

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SN - 9780819476609

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BT - 12th World Congress of the International Photodynamic Association - Photodynamic Therapy

T2 - 12th World Congress of the International Photodynamic Association - Photodynamic Therapy: Back to the Future

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ER -

Downregulation of the autophagy protein ATG-7 correlates with distinct sphingolipid profile in MCF-7 cells sensitized to photodamage

Abstract

Publication series

Other

UN SDGs

Keywords

Publisher link

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