Down-regulation of HCW9 mRNA in rat hepatocytes during chemical hypoxia involves both transcriptional and posttranscriptional mechanisms

Haichao Wang, D. Corinne Harrison-Shostak, John J. Lemasters, Brian Herman

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

HCW9 cDNA encodes a rat protein with 95% homology to mouse phospholipase A2 activating protein (PLAP). Its mRNA, which is substantially decreased in rat hepatocytes during chemical hypoxic injury, was found to be expressed in all rat tissues examined, including liver, heart, brain, spleen, lung, skeletal muscle, kidney, and testis. To elucidate the mechanisms responsible for this hypoxia-induced down-regulation of HCW9 mRNA levels, the transcription rate and half-life of HCW9 mRNA were measured. Nuclear run-off assays revealed a 54-57% inhibition in the transcription rate of HCW9 gene during chemical hypoxic injury. The half-life of HCW9 mRNA decreased from ~15 min under normoxic conditions to ~7 min during chemical hypoxic injury. These findings suggest that HCW9 expression in rat hepatocytes is regulated at both the transcriptional and posttranscriptional levels during chemical hypoxia.

Original languageEnglish (US)
Pages (from-to)360-364
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume218
Issue number1
DOIs
StatePublished - Jan 5 1996

Bibliographical note

Funding Information:
Expression and purification of HCW9 protein. The 1944-bp coding region (nucleotide 144–2087) of HCW9 clone (1) was amplified by PCR using primers (5′-CCC GCG GAT CCA TGC ACT GTA TGA GCG GCC, and 5′-CCC GCA AGC TTT 1This work was supported by Grant AG07218 from the National Institutes of Health. 2Corresponding Author: (919)966-1856 (Fax).

Fingerprint Dive into the research topics of 'Down-regulation of HCW9 mRNA in rat hepatocytes during chemical hypoxia involves both transcriptional and posttranscriptional mechanisms'. Together they form a unique fingerprint.

Cite this